Human chemokine (C-C motif) ligand 1 ELISA Kit | CCL1 elisa kit

Human C-C motif chemokine 1, CCL1 ELISA Kit

Cat. Num. AAA112379

• Gene Names: CCL1; P500; SISe; TCA3; I-309; SCYA1
• Reactivity: Human
• Synonyms: chemokine (C-C motif) ligand 1; N/A; Human C-C motif chemokine 1, CCL1 ELISA Kit; Human C-C motif chemokine 1 (CCL1) ELISA kit; I-309; P500; SCYA1; SISe; TCA3; T lymphocyte-secreted protein I-309; inflammatory cytokine I-309; small inducible cytokine A1; small inducible cytokine A1 (I-309; homologous to mouse Tca-3) ; CCL1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CCL1. No significant cross-reactivity or interference between human CCL1 and analogues was observed.
• Sequence Length: 96
• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 15.6 pg/ml -1000 pg/ml.
• Sensitivity: The minimum detectable dose of human CCL1 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for CCL1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CCL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4506833
• NCBI GeneID: 6346
• NCBI Accession #: NP_002972.1
• NCBI GenBank Nucleotide #: NM_002981.2
• UniProt Accession #: P22362; Q2M309; B2R5G9
• Molecular Weight: 10,992 Da
• NCBI Official Full Name: C-C motif chemokine 1
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 1
• NCBI Official Symbol: CCL1
• NCBI Official Synonym Symbols: P500; SISe; TCA3; I-309; SCYA1
• NCBI Protein Information: C-C motif chemokine 1; inflammatory cytokine I-309; T lymphocyte-secreted protein I-309; small inducible cytokine A1 (I-309, homologous to mouse Tca-3)
• UniProt Protein Name: C-C motif chemokine 1
• UniProt Gene Name: CCL1
• UniProt Synonym Gene Names: SCYA1
• UniProt Entry Name: CCL1_HUMAN