Standard Curve (Sample)
Human Eotaxin ELISA Kit | CCL11 elisa kit
Human Eotaxin PicoKine ELISA Kit
Gene Names
CCL11; SCYA11
Reactivity
Human
Synonyms
Eotaxin; N/A; Human Eotaxin PicoKine ELISA Kit; chemokine (C-C motif) ligand 11; C-C motif chemokine 11; CCL 11; CCL11; CCL11_HUMAN; Chemokine (C-C motif) ligand 11; Chemokine C C motif ligand 11; Chemokine CC Motif Ligand 11; Chemokine ligand 11; Eosinophil Chemotactic P; Eosinophil chemotactic protein; Eotaxin 1; MGC22554; SCYA 11; SCYA11; Small inducible cytokine A11; Small inducible cytokine subfamily A (Cys Cys) member 11; Small inducible cytokine subfamily A member 11; Small-inducible cytokine A11; CCL11 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human CCL11
Sequence Length
97
Samples
Cell culture supernates, serum and plasma(citrate, edta)
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
<2pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess interassay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CCL11 elisa kit
Principle of the Assay: The Picokine™ Human CCL11 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CCL11 with a 96-well strip plate that is pre-coated with antibody specific for CCL11. The detection antibody is a biotinylated antibody specific for CCL11. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human CCL11 with immunogen: Expression system for standard: E.coli; Immunogen sequence: G24-P97. The kit is analytically validated with ready to use reagents. To measure Human CCL11, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human CCL11 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CCL11 in the sample.
Background/Introduction: Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3(CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin. Recombinant human eotaxin produced in insect cells induces a calcium flux response in normal human eosinophils, but not in neutrophils or monocytes. The human eotaxin gene is cloned and found to be 61.8 and 63.2% identical at the amino acid level to guinea pig and mouse eotaxin.
Background/Introduction: Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3(CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin. Recombinant human eotaxin produced in insect cells induces a calcium flux response in normal human eosinophils, but not in neutrophils or monocytes. The human eotaxin gene is cloned and found to be 61.8 and 63.2% identical at the amino acid level to guinea pig and mouse eotaxin.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
10,732 Da
NCBI Official Full Name
Eotaxin
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 11
NCBI Official Symbol
CCL11
NCBI Official Synonym Symbols
SCYA11
NCBI Protein Information
eotaxin; eotaxin-1; eosinophil chemotactic protein; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
UniProt Protein Name
Eotaxin
UniProt Gene Name
CCL11
UniProt Synonym Gene Names
SCYA11
UniProt Entry Name
CCL11_HUMAN
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Product Notes
The Human CCL11 ccl11 (Catalog #AAA45204) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45204 ELISA Kit recognizes Human CCL11. It is sometimes possible for the material contained within the vial of "Eotaxin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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