Mouse M-CSF ELISA Kit | CCL3 elisa kit

Mouse M-CSF PicoKine ELISA Kit

Cat. Num. AAA45261

• Gene Names: Csf1; op; Csfm; MCSF; C87615
• Reactivity: Mouse
• Synonyms: M-CSF; N/A; Mouse M-CSF PicoKine ELISA Kit; Macrophage colony-stimulating factor 1; chemokine (C-C motif) ligand 3; CSF-1; CSF1; Lanimostim; MCSF; Processed macrophage colony-stimulating factor 1; CCL3 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse M-CSF
• Sequence Length: 552
• Samples: Cell culture supernates, tissue homogenates, serum and plasma (EDTA)
• Assay Type: Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: < 1pg/ml

• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Mouse M-CSF PicoKine ELISA Kit standard curve)

Related Product Information for CCL3 elisa kit

Principle of the assay: mouse M-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for M-CSF has been precoated onto 96-well plates. Standards (E.coli,K33-E262) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for M-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse M-CSF amount of sample captured in plate.
Background: M-CSF, also called CSF1, consists of a 14-amino acid peptide, longer than the usual 8-to-11 mer recognized by most CTLs. The M-CSF gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb. Although it is a single-copy gene, its expression results in the synthesis of several mRNAs, ranging in size from about 1.5 to 4.5 kb. There are 2 forms of M-CSF, with 224 and 522 amino acids, resulting from alternative splicing. Furthermore, M-CSF has a role in development of the placenta. Uterine M-CSF concentration is regulated by a synergistic action of estradiol and progesterone. M-CSF is produced by uterine glandular epithelial cells. It had been found that FMS, the M-CSF receptor, is expressed in placenta and choriocarcinoma cell lines.

NCBI and Uniprot Product Information

• NCBI GI #: 1345843
• NCBI GeneID: 12977
• UniProt Accession #: P07141; Q3U395; Q8R3C8
• Molecular Weight: 29,353 Da
• NCBI Official Full Name: Macrophage colony-stimulating factor 1
• NCBI Official Synonym Full Names: colony stimulating factor 1 (macrophage)
• NCBI Official Symbol: Csf1
• NCBI Official Synonym Symbols: op; Csfm; MCSF; C87615
• NCBI Protein Information: macrophage colony-stimulating factor 1; osteopetrosis
• UniProt Protein Name: Macrophage colony-stimulating factor 1
• UniProt Gene Name: Csf1
• UniProt Synonym Gene Names: Csfm; CSF-1; MCSF
• UniProt Entry Name: CSF1_MOUSE