Mouse chemokine (C-C motif) ligand 5 ELISA Kit | CCL5 elisa kit

Mouse regulated on activation in normal T-cell expressed and secreted, RANTES ELISA Kit

Cat. Num. AAA79869

• Gene Names: Ccl5; SISd; Scya5; RANTES; TCP228; MuRantes
• Reactivity: Mouse
• Synonyms: chemokine (C-C motif) ligand 5; N/A; Mouse regulated on activation in normal T-cell expressed and secreted, RANTES ELISA Kit; Mouse regulated on activation in normal T-cell expressed and secreted; RANTES ELISA kit; D17S136E; MGC17164; RANTES; SCYA5; SISd; TCP228; OTTHUMP00000197106; SIS-delta; T-cell specific protein p288; beta-chemokine RANTES; regulated upon activation; normally T-expressed; and presumably secre; CCL5 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse CCL5. No significant cross-reactivity or interference between Mouse CCL5 and analogues was observed.
• Sequence Length: 91
• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 62.5 pg/ml-4000 pg/ml
• Sensitivity: 15.6 pg/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Immunity
• Protein Biological Process 2: chemokine
• Protein Biological Process 3: Chemotaxis
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for CCL5 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RANTES has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RANTES present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RANTES is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RANTES bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 164698428
• NCBI GeneID: 20304
• NCBI Accession #: NP_038681.2
• NCBI GenBank Nucleotide #: NM_013653.3
• UniProt Accession #: P30882
• Molecular Weight: 10,071 Da
• NCBI Official Full Name: C-C motif chemokine 5
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 5
• NCBI Official Symbol: Ccl5
• NCBI Official Synonym Symbols: SISd; Scya5; RANTES; TCP228; MuRantes
• NCBI Protein Information: C-C motif chemokine 5; SIS-delta; small inducible cytokine A5; small-inducible cytokine A5; T-cell-specific protein RANTES
• UniProt Protein Name: C-C motif chemokine 5
• UniProt Gene Name: Ccl5
• UniProt Synonym Gene Names: Scya5
• UniProt Entry Name: CCL5_MOUSE