Standard Curve (Sample)
Mouse Cluster of differentiation 100 ELISA Kit | CD100 elisa kit
Mouse Cluster of differentiation 100 ELISA Kit
Gene Names
SEMA4D; CD100; SEMAJ; coll-4; C9orf164; M-sema-G
Reactivity
Mouse
Synonyms
Cluster of differentiation 100; N/A; Mouse Cluster of differentiation 100 ELISA Kit; CD100 elisa kit
Reactivity
Mouse
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
0.5-10ng/ML
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CD100 elisa kit
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This CD100 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CD100. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY CD100 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CD100. Standards or samples are then added to the microtiter plate wells and CD100 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CD100 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CD100 are added to each well to “sandwich” the CD100 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzym e(HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CD100 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD100 concentration in each sample is interpolated from this standard curve.
INTENDED USE This CD100 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CD100. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY CD100 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CD100. Standards or samples are then added to the microtiter plate wells and CD100 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CD100 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CD100 are added to each well to “sandwich” the CD100 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzym e(HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CD100 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD100 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CD100 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
82,164 Da
NCBI Official Full Name
semaphorin-4D isoform 1
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
NCBI Official Symbol
SEMA4D
NCBI Official Synonym Symbols
CD100; SEMAJ; coll-4; C9orf164; M-sema-G
NCBI Protein Information
semaphorin-4D; A8; GR3; BB18; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D
UniProt Protein Name
Semaphorin-4D
UniProt Gene Name
SEMA4D
UniProt Synonym Gene Names
C9orf164; CD100; SEMAJ
UniProt Entry Name
SEM4D_HUMAN
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Product Notes
The Mouse CD100 sema4d (Catalog #AAA84464) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA84464 ELISA Kit recognizes Mouse CD100. It is sometimes possible for the material contained within the vial of "Cluster of differentiation 100, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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