Application Data
(Immobilized Recombinant Mouse 2B4/CD244 Protein (ECD, His Tag)(#AAA259390) at 2ug/mL (100uL/well) can bind Recombinant Mouse CD48/SLAMF2/BCM1 Protein (Fc Tag), the EC50is 180-550ng/mL.)
2B4/CD244 Recombinant Protein | CD244 recombinant protein
Recombinant Mouse 2B4/CD244 Protein (ECD, His Tag), HPLC-verified
Gene Names
Cd244; 2B4; C9.1; Ly90; NAIL; Nmrk; NKR2B4; SLAMF4; F730046O15Rik
Reactivity
Mouse
Purity
>= 95% as determined by SDS-PAGE. >= 95% as determined by SEC-HPLC.
Synonyms
2B4/CD244; N/A; Recombinant Mouse 2B4/CD244 Protein (ECD, His Tag), HPLC-verified; Mouse 2B4/CD244 Protein (ECD, His Tag); CD244 molecule, natural killer cell receptor 2B4; 2b4 Protein, Mouse; Cd244 Protein, Mouse; Nmrk Protein, Mouse; CD244 recombinant protein
Host
HEK293 Cells
Reactivity
Mouse
Purity/Purification
>= 95% as determined by SDS-PAGE. >= 95% as determined by SEC-HPLC.
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Sequence
Met1-Pro226
Tag
C-His
Predicted N Terminal
Gln 20
Endotoxin
< 1.0 EU perug protein as determined by the LAL method.
Protein Construction
A DNA sequence encoding the Mouse CD244 (NP_061199.2)(Met1-Pro226) was expressed with a polyhistidine tag at the C-terminus.
Activity
Immobilized Recombinant Mouse 2B4/CD244 Protein (ECD, His Tag) at 2ug/mL (100uL/well) can bind Recombinant Mouse CD48/SLAMF2/BCM1 Protein (Fc Tag), the EC50is 180-550ng/mL.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Application Data
(Immobilized Recombinant Mouse 2B4/CD244 Protein (ECD, His Tag)(#AAA259390) at 2ug/mL (100uL/well) can bind Recombinant Mouse CD48/SLAMF2/BCM1 Protein (Fc Tag), the EC50is 180-550ng/mL.)
Application Data
(Immobilized Recombinant Mouse 2B4/CD244 Protein (ECD, His Tag)(#AAA259390) at 2ug/mL (100uL/well) can bind Recombinant Mouse CD48/SLAMF2/BCM1 Protein (Fc Tag), the EC50is 180-550ng/mL.)
Application Data
Application Data
Application Data
Application Data
Related Product Information for CD244 recombinant protein
The CD244 antigen, also known as 2B4, is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating NK cell cytotoxicity and cytokine production, which is expressed on all NK cells, a subpopulation of T cells, monocytes and basophils. The 2B4 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor, human 2B4 can effectively activate and enhance NK cell-mediated cytotoxicity, induce secretion of IFN-gamma and matrix metalloproteinases (MMPs), as well as NK cell invasiveness. As a cell surface glycoprotein of the Ig-superfamily structurally related to CD2-like molecules such as CD2, CD48, CD58, CD84, Ly-9, and SLAM, 2B4 (CD244) is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. 2B4 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness.
Product Categories/Family for CD244 recombinant protein
References
Nakajim. H. e. al. (2000. 2B4. a. N. cel. activatin. recepto. wit. uniqu. specificit. an. signa. transductio. mechanism. Hu. Immunol. 61(1). 39-43.Chuan. SS. e. al. (2001. 2B. (CD244)-mediate. activatio. o. cytotoxicit. an. IFN-gamm. releas. i. huma. N. cell. involve. distinc. pathways. . Immunol. 167(11). 6210-6.McNerne. ME. e. al. (2005. 2B. (CD244. i. . non-MH. bindin. recepto. wit. multipl. function. o. natura. kille. cell. an. CD8. . cells. Mo. Immunol. 42(4). 489-94.Mathe. SO. e. al. (2009. Functiona. rol. o. huma. N. cel. recepto. 2B. (CD244. isoforms. Eu. . Immunol. 39(6). 1632-41.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,836 Da
NCBI Official Full Name
natural killer cell receptor 2B4
NCBI Official Synonym Full Names
CD244 natural killer cell receptor 2B4
NCBI Official Symbol
Cd244
NCBI Official Synonym Symbols
2B4; C9.1; Ly90; NAIL; Nmrk; NKR2B4; SLAMF4; F730046O15Rik
NCBI Protein Information
natural killer cell receptor 2B4; NK cell receptor 2B4; SLAM family member 4; NK cell type I receptor protein 2B4; non MHC restricted killing associated; non-MHC restricted killing associated; signaling lymphocytic activation molecule 4
UniProt Protein Name
Natural killer cell receptor 2B4
UniProt Gene Name
Cd244
UniProt Synonym Gene Names
2b4; Nmrk; NKR2B4; SLAMF4
UniProt Entry Name
CD244_MOUSE
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Product Notes
The CD244 cd244 (Catalog #AAA259390) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Mouse 2B4/CD244 Protein (ECD, His Tag), HPLC-verified reacts with Mouse and may cross-react with other species as described in the data sheet. The amino acid sequence is listed below: Met1-Pro22 6. It is sometimes possible for the material contained within the vial of "2B4/CD244, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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