CD27/TNFRSF7 recombinant protein

Recombinant human CD27/TNFRSF7 protein

Cat. Num. AAA48413

• Gene Names: CD27; T14; S152; Tp55; TNFRSF7; S152. LPFS2
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: CD27/TNFRSF7; N/A; Recombinant human CD27/TNFRSF7 protein; CD27 antigen; CD27L receptor; T-cell activation antigen CD27; S152; LPFS2; T14; TNFRSF7; Tp55; CD27 molecule; CD 27; CD27_HUMAN; TNFRSF 7; CD27/TNFRSF7 recombinant protein

• Host: HEK293
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 20-191aa
• Sequence: ATPAPKSCPE RHYWAQGKLC CQMCEPGTFL VKDCDQHRKA AQCDPCIPGV SFSPDHHTRP HCESCRHCNS GLLVRNCTIT ANAECACRNG WQCRDKECTE CDPLPNPSLT ARSSQALSPH PQPTHLPYVS EMLEARTAGH MQTLADFRQL PARTLSTHWP PQRSLCSSDF IR

• Applicable Applications for CD27/TNFRSF7 recombinant protein: Bioactivity, SDS-PAGE
• Species: Human
• Tag: hIgG-His-Tag
• Predicted Nterm: Ala 20
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Bioactivity: Measured by its binding ability in a functional ELISA with Human CD27 Ligand/TNFSF7. The ED50 range <= 0.8 ug/ml.
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bioactivity

(Human CD27 Ligand/TNFSF7 is coated at 10 ug/ml (100 ul/well) can bind Human CD27/TNFRSF7. The ED50 range )

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for CD27/TNFRSF7 recombinant protein

CD27, as known as TNFRSF7, is a member of the tumor necrosis factor receptor superfamily. It has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has an important role in the generation of T cell immunity and is an robust marker for normal memory B cells. Also, this protein plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD 70 on lymphocytes and dendritic cells. It binds to ligand CD70, and CD27/CD70 co-stimulatory system promote T, B and NK cell responses and particularly the CTL response, and is a target in cancer immunotherapy. Recombinant human CD27, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

Product Categories/Family for CD27/TNFRSF7 recombinant protein

Immunology

References

Clemente A., et al. (2013) Clin. Exp. Immunol. 174:97-108.;; Huang J., et al. (2013) J. Immunol. 190:6250-6258.

NCBI and Uniprot Product Information

• NCBI GI #: 4507587
• NCBI GeneID: 939
• NCBI Accession #: NP_001233.1
• NCBI GenBank Nucleotide #: NM_001242.4
• UniProt Accession #: P26842; B2RDZ0
• Molecular Weight: 29,137 Da
• NCBI Official Full Name: CD27 antigen
• NCBI Official Synonym Full Names: CD27 molecule
• NCBI Official Symbol: CD27
• NCBI Official Synonym Symbols: T14; S152; Tp55; TNFRSF7; S152. LPFS2
• NCBI Protein Information: CD27 antigen; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7
• UniProt Protein Name: CD27 antigen
• UniProt Gene Name: CD27
• UniProt Synonym Gene Names: TNFRSF7
• UniProt Entry Name: CD27_HUMAN