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product-image-AAA47195_SDS_PAGE13.jpg SDS-PAGE (Figure 1. Human CD27 Protein, mFc-His Tag on SDS-PAGE under reducing condition.)

CD27 recombinant protein

Recombinant human CD27 protein with C-terminal mouse Fc and 6xHis tag

Gene Names
CD27; T14; S152; Tp55; TNFRSF7; S152. LPFS2
Applications
Western Blot
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Synonyms
CD27; N/A; Recombinant human CD27 protein with C-terminal mouse Fc and 6xHis tag; Human CD27 Protein, mFc-His Tag; TNFRSF7; S152; T14; Tp55; CD27 recombinant protein
Ordering
Host
HEK293 cells
Purity/Purification
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5%-20% trehalose is added as protectants before lyophilization.
Sequence
CD27(Ala20-Arg191)+mFc(Pro99-Lys330)
Applicable Applications for CD27 recombinant protein
WB (Western Blot)
Source
Human
Conjugation
C-Mouse Fc and 6xHis tag
Reconstitution
Reconstitute with deionized water
Preparation and Storage
Store at -80 degree C for 12 months (Avoid repeated freezing and thawing)
Shipping: Blue Ice

SDS-PAGE

(Figure 1. Human CD27 Protein, mFc-His Tag on SDS-PAGE under reducing condition.)

product-image-AAA47195_SDS_PAGE13.jpg SDS-PAGE (Figure 1. Human CD27 Protein, mFc-His Tag on SDS-PAGE under reducing condition.)

ELISA

(Figure 1. Human CD27 Protein, mFc-His Tag on SDS-PAGE under reducing condition.)

product-image-AAA47195_ELISA15.png ELISA (Figure 1. Human CD27 Protein, mFc-His Tag on SDS-PAGE under reducing condition.)
Related Product Information for CD27 recombinant protein
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
939
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,137 Da
NCBI Official Full Name
CD27 antigen
NCBI Official Synonym Full Names
CD27 molecule
NCBI Official Symbol
CD27
NCBI Official Synonym Symbols
T14; S152; Tp55; TNFRSF7; S152. LPFS2
NCBI Protein Information
CD27 antigen; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7
UniProt Protein Name
CD27 antigen
UniProt Gene Name
CD27
UniProt Synonym Gene Names
TNFRSF7
UniProt Entry Name
CD27_HUMAN

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Product Notes

The CD27 cd27 (Catalog #AAA47195) is a Recombinant Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD27 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the CD27 cd27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CD27(Ala20 -Arg191)+m Fc(Pro99-L ys330). It is sometimes possible for the material contained within the vial of "CD27, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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