SDS-PAGE
(Figure 1. Human B7-H3 Protein, mFc-His Tag on SDS-PAGE under reducing condition.)
B7-H3 recombinant protein
Recombinant human B7-H3 protein with C-terminal mouse Fc and 6xHis tag
Gene Names
CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
Applications
ELISA, Western Blot
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Synonyms
B7-H3; N/A; Recombinant human B7-H3 protein with C-terminal mouse Fc and 6xHis tag; Human B7-H3 Protein, mFc-His Tag; CD276; B7 homolog 3; B7-H3 recombinant protein
Host
HEK293 cells
Purity/Purification
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5%-19% trehalose is added as protectants before lyophilization.
Sequence
B7-H3(Leu29-Pro245)+mFc(Pro99-Lys330)
Applicable Applications for B7-H3 recombinant protein
ELISA, WB (Western Blot)
Source
Human
Conjugation
C-Mouse Fc and 6xHis tag
Reconstitution
Reconstitute with deionized water
Preparation and Storage
Store at -80 degree C for 12 months (Avoid repeated freezing and thawing)
Shipping: Blue Ice
Shipping: Blue Ice
SDS-PAGE
(Figure 1. Human B7-H3 Protein, mFc-His Tag on SDS-PAGE under reducing condition.)
SDS-PAGE
(Figure 1. Human B7-H3 Protein, mFc-His Tag on SDS-PAGE under reducing condition.)
ELISA
(Figure 1. Human B7-H3 Protein, mFc-His Tag on SDS-PAGE under reducing condition.)
ELISA
(Figure 1. Human B7-H3 Protein, mFc-His Tag on SDS-PAGE under reducing condition.)
ELISA
(Figure 1. Human B7-H3 Protein, mFc-His Tag on SDS-PAGE under reducing condition.)
ELISA
(Figure 1. Human B7-H3 Protein, mFc-His Tag on SDS-PAGE under reducing condition.)
Related Product Information for B7-H3 recombinant protein
The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
57,235 Da
NCBI Official Full Name
B7-H3 protein
NCBI Official Synonym Full Names
CD276 molecule
NCBI Official Symbol
CD276
NCBI Official Synonym Symbols
B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
NCBI Protein Information
CD276 antigen; B7 homolog 3; costimulatory molecule
UniProt Protein Name
CD276 antigen
UniProt Gene Name
CD276
UniProt Synonym Gene Names
B7H3; B7-H3
UniProt Entry Name
CD276_HUMAN
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Product Notes
The B7-H3 cd276 (Catalog #AAA47194) is a Recombinant Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's B7-H3 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the B7-H3 cd276 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: B7-H3(Leu2 9-Pro245)+ mFc(Pro99- Lys330). It is sometimes possible for the material contained within the vial of "B7-H3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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