FCM/FACS (Flow Cytometry)
(Figure 1. 1e5 of Expi 293 cell line were stained with 100 uL of 1:1000 diluted Biotinylated Anti-B7-H3 antibody (DM53), Rabbit mAb (Blue histogram) or isotype control (Red histogram) respectively, washed and then stained with Streptavidin APC. The experimental samples were analyzed by flow cytometry.)
Rabbit anti-Human B7-H3 Monoclonal Antibody | anti-B7-H3 antibody
Biotinylated Anti-B7-H3 antibody (DM53), Rabbit mAb
Gene Names
CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
Purified from cell culture supernatant by affinity chromatography
Synonyms
B7-H3, Antibody; Biotinylated Anti-B7-H3 antibody (DM53), Rabbit mAb; B7-H3; CD276; B7 homolog 3; B7H3; anti-B7-H3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
DM53
Purity/Purification
Purified from cell culture supernatant by affinity chromatography
Form/Format
Powder.
Buffer:
Preservative 0.1% Procline 300
Constituents: PBS, pH7.4
Buffer:
Preservative 0.1% Procline 300
Constituents: PBS, pH7.4
Applicable Applications for anti-B7-H3 antibody
FCM/FACS (Flow Cytometry), ELISA
Immunogen
Recombinant human B7H3(Leu29-Pro245) produced by using human HEK293 cells
Reconstitution
Reconstitute with deionized water
Preparation and Storage
Store at 2-8 degree C.
FCM/FACS (Flow Cytometry)
(Figure 1. 1e5 of Expi 293 cell line were stained with 100 uL of 1:1000 diluted Biotinylated Anti-B7-H3 antibody (DM53), Rabbit mAb (Blue histogram) or isotype control (Red histogram) respectively, washed and then stained with Streptavidin APC. The experimental samples were analyzed by flow cytometry.)
FCM/FACS (Flow Cytometry)
(Figure 1. 1e5 of Expi 293 cell line were stained with 100 uL of 1:1000 diluted Biotinylated Anti-B7-H3 antibody (DM53), Rabbit mAb (Blue histogram) or isotype control (Red histogram) respectively, washed and then stained with Streptavidin APC. The experimental samples were analyzed by flow cytometry.)
Related Product Information for anti-B7-H3 antibody
The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
B7-H3 protein
NCBI Official Synonym Full Names
CD276 molecule
NCBI Official Symbol
CD276
NCBI Official Synonym Symbols
B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
NCBI Protein Information
CD276 antigen; B7 homolog 3; costimulatory molecule
UniProt Protein Name
CD276 antigen
UniProt Gene Name
CD276
UniProt Synonym Gene Names
B7H3; B7-H3
UniProt Entry Name
CD276_HUMAN
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Product Notes
The B7-H3 cd276 (Catalog #AAA47459) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Biotinylated Anti-B7-H3 antibody (DM53), Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's B7-H3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the B7-H3 cd276 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B7-H3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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