SDS-PAGE
(Figure 1. Human CD33, hFc-His Tag on SDS-PAGE under reducing condition.)
CD33 recombinant protein
Recombinant human CD33 protein with C-terminal human Fc and 6xHis tag
Gene Names
CD33; p67; SIGLEC3; SIGLEC-3
Applications
Western Blot
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Synonyms
CD33; N/A; Recombinant human CD33 protein with C-terminal human Fc and 6xHis tag; Human CD33 Protein, hFc-His Tag; CD33;SIGLEC3;gp67; CD33 recombinant protein
Host
HEK293 cells
Purity/Purification
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5%-44% trehalose is added as protectants before lyophilization.
Sequence
CD33(Asp18-His259)+hFc(Glu99-Ala330)
Applicable Applications for CD33 recombinant protein
WB (Western Blot)
Source
Human
Conjugation
C-Human Fc and 6xHis tag
Reconstitution
Reconstitute with deionized water
Preparation and Storage
Store at -80 degree C for 12 months (Avoid repeated freezing and thawing)
Shipping: Blue Ice
Shipping: Blue Ice
SDS-PAGE
(Figure 1. Human CD33, hFc-His Tag on SDS-PAGE under reducing condition.)
SDS-PAGE
(Figure 1. Human CD33, hFc-His Tag on SDS-PAGE under reducing condition.)
ELISA
(Figure 1. Human CD33, hFc-His Tag on SDS-PAGE under reducing condition.)
ELISA
(Figure 1. Human CD33, hFc-His Tag on SDS-PAGE under reducing condition.)
Related Product Information for CD33 recombinant protein
Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3-and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Calculated MW: 25kDa/33kDa/39kDa
Observed MW: 77kDa
Observed MW: 77kDa
NCBI Official Full Name
Myeloid cell surface antigen CD33
NCBI Official Synonym Full Names
CD33 molecule
NCBI Official Symbol
CD33
NCBI Official Synonym Symbols
p67; SIGLEC3; SIGLEC-3
NCBI Protein Information
myeloid cell surface antigen CD33; gp67; CD33 antigen (gp67); sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3
UniProt Protein Name
Myeloid cell surface antigen CD33
UniProt Gene Name
CD33
UniProt Synonym Gene Names
SIGLEC3; Siglec-3
UniProt Entry Name
CD33_HUMAN
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Product Notes
The CD33 cd33 (Catalog #AAA47219) is a Recombinant Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD33 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the CD33 cd33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CD33(Asp18 -His259)+h Fc(Glu99-A la330). It is sometimes possible for the material contained within the vial of "CD33, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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