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Application Data
(Biotinylated Human CD40/TNFRSF5 Protein Blocking DataSerial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml.)
CD40/TNFRSF5 recombinant protein
Biotinylated Human CD40/TNFRSF5 Protein Recombinant
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
CD40/TNFRSF5; N/A; Biotinylated Human CD40/TNFRSF5 Protein Recombinant; CD40/TNFRSF5 recombinant protein
Host
HEK293 cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant Human CD40/TNFRSF5 was lyophilized from 0.2 um filtered PBS solution pH7.4
Source
DNA sequence encoding Human CD40/TNFRSF5 including a C-His-Avi tag was expressed in HEK293 Cells.The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Molecular Function
Receptor
Biological Process
Immunity
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at-20 degree C.
Application Data
(Biotinylated Human CD40/TNFRSF5 Protein Blocking DataSerial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml.)
Application Data
(Biotinylated Human CD40/TNFRSF5 Protein Blocking DataSerial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml.)
Application Data
(Biotinylated Human CD40/TNFRSF5 Protein ELISA DataImmobilized Biotinylated Human CD40, His Tag at 1?g/ml (100?l/well) on the plate. Dose response curve for Anti-CD40 Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.)
Application Data
(Biotinylated Human CD40/TNFRSF5 Protein ELISA DataImmobilized Biotinylated Human CD40, His Tag at 1?g/ml (100?l/well) on the plate. Dose response curve for Anti-CD40 Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.)
ELISA
(Biotinylated Human CD40/TNFRSF5 Protein ELISA DataImmobilized Biotinylated Human CD40, His Tag at 1?g/ml (100?l/Well) on the plate. Dose response curve for Human CD40L, hFc Tag with the EC50 of 33ng/ml determined by ELISA.)
ELISA
(Biotinylated Human CD40/TNFRSF5 Protein ELISA DataImmobilized Biotinylated Human CD40, His Tag at 1?g/ml (100?l/Well) on the plate. Dose response curve for Human CD40L, hFc Tag with the EC50 of 33ng/ml determined by ELISA.)
Application Data
(Biotinylated Human CD40/TNFRSF5 Protein SEC-HPLCThe purity of Biotinylated Human CD40 is greater than 95% as determined by SEC-HPLC.)
Application Data
(Biotinylated Human CD40/TNFRSF5 Protein SEC-HPLCThe purity of Biotinylated Human CD40 is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE
(Biotinylated Human CD40/TNFRSF5 Protein Tris-Bis PAGEBiotinylated Human CD40 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
Tris-Bis-PAGE
(Biotinylated Human CD40/TNFRSF5 Protein Tris-Bis PAGEBiotinylated Human CD40 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
Product Categories/Family for CD40/TNFRSF5 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,619 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 5 isoform 1
NCBI Official Synonym Full Names
CD40 molecule, TNF receptor superfamily member 5
NCBI Official Symbol
CD40
NCBI Official Synonym Symbols
p50; Bp50; CDW40; TNFRSF5
NCBI Protein Information
tumor necrosis factor receptor superfamily member 5; CD40L receptor; CD40 type II isoform; B cell-associated molecule; B cell surface antigen CD40; B-cell surface antigen CD40; CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor recept
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 5
UniProt Gene Name
CD40
UniProt Synonym Gene Names
TNFRSF5
UniProt Entry Name
TNR5_HUMAN
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Product Notes
The CD40/TNFRSF5 cd40 (Catalog #AAA76149) is a Recombinant Protein produced from HEK293 cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CD40/TNFRSF5, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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