Standard Curve (Sample)
Mouse CD154 ELISA Kit | CD40LG elisa kit
Mouse CD154 ELISA Kit
Gene Names
Cd40lg; IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Tnlg8b
Reactivity
Mouse
Applications
ELISA
Synonyms
CD154; N/A; Mouse CD154 ELISA Kit; CD40L; TNFSF5; TRAP; CD40 ligand; CD40-L; T-cell antigen Gp39; TNF-related activation protein; Tumor necrosis factor ligand superfamily member 5; CD40LG elisa kit
Reactivity
Mouse
Specificity
The Mouse CD154 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CD154 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
Sequence Length
260
Samples
Cell Culture Supernatants, Serum, Plasma.
Sensitivity
10 pg/ml.
Applicable Applications for CD40LG elisa kit
ELISA (Sandwich ELISA)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CD40LG elisa kit
Principle of the Assay||The Mouse CD154 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD154 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CD154 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD154 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD154 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD154 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: CD40 ligand (CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb/IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.
Background/Introduction: CD40 ligand (CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb/IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
29,370 Da
NCBI Official Full Name
CD40 ligand
NCBI Official Synonym Full Names
CD40 ligand
NCBI Official Symbol
Cd40lg
NCBI Official Synonym Symbols
IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Tnlg8b
NCBI Protein Information
CD40 ligand
UniProt Protein Name
CD40 ligand
UniProt Gene Name
Cd40lg
UniProt Synonym Gene Names
Cd40l; Tnfsf5; CD40-L; TRAP
UniProt Entry Name
CD40L_MOUSE
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Product Notes
The Mouse CD40LG cd40lg (Catalog #AAA261694) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA261694 ELISA Kit recognizes Mouse CD40LG. AAA Biotech's CD154 can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the CD40LG cd40lg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD154, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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