CD40 Ligand recombinant protein

Human CD40 Ligand Recombinant

Cat. Num. AAA76087

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: CD40 Ligand; N/A; Human CD40 Ligand Recombinant; cd40l; cd40 ligand; trap; cd40lg; CD154; TNFSF5; CD40 Ligand recombinant protein

• Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Sequence Length: 261

• Source: Optimized DNA sequence encoding Human CD40L TNF domain was expressed in Escherichia Coli.
• Biological Activity: The ED(50) was determined by the dose-dependent stimulation of IL-8 production by human PBMC and was found to be in the range of 5.0-20.0ng/ml.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
• Presentation: CD40 ligand was lyophilized from a 0.2 um filtered PBS solution pH 7.0.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2°-8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity.; Avoid repeated freeze/thaw cycles.

Related Product Information for CD40 Ligand recombinant protein

CD40 protein is expressed on all B-lymphocytes during various stages of development, activated T- cells and monocytes, follicular dendritic cells, thymic epithelial cells, and various carcinoma cell lines. It is expressed on most mature B-cell malignancies and on some early B-cell acute lymphocytic leukemias. Human CD40 has been found to be the receptor for a protein involved in an X- linked immunodeficiency syndrome. The CD40 ligand is identical with a T-cell restricted cell surface activation antigen designated 5c8. The mouse ligand of CD40 is gp39.Based on its homology with other members of the TNF ligand protein superfamily the protein is referred to also as TNFSF5.

Product Categories/Family for CD40 Ligand recombinant protein

Cytokines

References

• the cd40/CD40 Ligand system: linking inflammation with atherothrombosis .J. Am. Coll. Cardiol., Aug 2009; 54: 669 - 677.
• growth inhibition of human multiple myeloma cells by an oncolytic adenovirus carrying the CD40 Ligand transgene .Clin. Cancer Res., Aug 2009; 15: 4847 - 4856.
• matrix metalloproteinase 9 is involved in crohn’s disease-associated platelet hyperactivation through the release of soluble CD40 Ligand .Gut, Jul 2009; 58: 920 - 928.
• distinct roles of cd28- and CD40 Ligand-mediated costimulation in the development of protective immunity and pathology during chlamydia muridarum urogenital infection in mice .Infect. Immun., Jul 2009; 77: 3080 - 3089.
• phase i study of intranodal direct injection of adenovirus encoding recombinant cd40-ligand (ad-isf35) in patients with chronic lymphocytic leukemia .ASCO Meeting Abstracts, May 2009; 27: 3003.