SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
CDC25C recombinant protein
CDC25C, Active
Gene Names
CDC25C; CDC25; PPP1R60
Applications
Western Blot
Synonyms
CDC25C; N/A; CDC25C, Active; CDC25; CDC25C recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 20mM MOPS, pH7.5, 300mM NaCl, 10mM glutathione, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for CDC25C recombinant protein
WB (Western Blot), Phosphatase Assay
Tag
GST
Species
Human
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet.)
Related Product Information for CDC25C recombinant protein
Full-length recombinant human CDC25C was expressed in Sf9 insect cells using an N-terminal GST tag. The gene accession number is BC019089.
CDC25C (also known as cell division cycle 25 homolog C) is a member of the CDC25 phosphatase family. CDC25C is highly conserved during evolution and it plays a key role in the regulation of cell division. CDC25C activates the partially purified p34 (cdc2)/cyclin B complex and directs the dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis (1). CDC25C also suppresses p53-induced growth arrest. The regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation (2).
CDC25C (also known as cell division cycle 25 homolog C) is a member of the CDC25 phosphatase family. CDC25C is highly conserved during evolution and it plays a key role in the regulation of cell division. CDC25C activates the partially purified p34 (cdc2)/cyclin B complex and directs the dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis (1). CDC25C also suppresses p53-induced growth arrest. The regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation (2).
Product Categories/Family for CDC25C recombinant protein
References
1. Gould, K. L. et.al: Complementation of the mitotic activator, p80(cdc25), by a human protein-tyrosine phosphatase. Science 250: 1573-1576, 1990.
2. Bulavin, D. V. et.al: Initiation of a G2/M checkpoint after ultraviolet radiation requires p38 kinase. Nature 411: 102-107, 2001.
2. Bulavin, D. V. et.al: Initiation of a G2/M checkpoint after ultraviolet radiation requires p38 kinase. Nature 411: 102-107, 2001.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
45,608 Da
NCBI Official Full Name
Homo sapiens cell division cycle 25 homolog C (S. pombe), mRNA
NCBI Official Synonym Full Names
cell division cycle 25C
NCBI Official Symbol
CDC25C
NCBI Official Synonym Symbols
CDC25; PPP1R60
NCBI Protein Information
M-phase inducer phosphatase 3
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Product Notes
The CDC25C (Catalog #AAA73287) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CDC25C can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Phosphatase Assay. Researchers should empirically determine the suitability of the CDC25C for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC25C, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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