Standard Curve (Sample)
Canine Cadherin 5 ELISA Kit | CDH5 elisa kit
Canine Cadherin 5 ELISA Kit
Gene Names
CDH5; 7B4; CD144
Reactivity
Canine
Synonyms
Cadherin 5; N/A; Canine Cadherin 5 ELISA Kit; CDH5 elisa kit
Reactivity
Canine
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CDH5 elisa kit
Intended Uses: This CDC5L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanCDC5L. This ELISA kit for research use only!
Principle of the Assay: CDC5L ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CDC5L antibody and an CDC5L-HRP conjugate. The assay sample and buffer are incubated together with CDC5L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDC5L concentration since CDC5L from samples and CDC5L-HRP conjugate compete for the anti-CDC5L antibody binding site. Since the number of sites is limited, as more sites are occupied by CDC5L from the sample, fewer sites are left to bind CDC5L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDC5L concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CDC5L ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CDC5L antibody and an CDC5L-HRP conjugate. The assay sample and buffer are incubated together with CDC5L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDC5L concentration since CDC5L from samples and CDC5L-HRP conjugate compete for the anti-CDC5L antibody binding site. Since the number of sites is limited, as more sites are occupied by CDC5L from the sample, fewer sites are left to bind CDC5L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDC5L concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CDH5 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,499 Da
NCBI Official Full Name
cadherin-5 preproprotein
NCBI Official Synonym Full Names
cadherin 5, type 2 (vascular endothelium)
NCBI Official Symbol
CDH5
NCBI Official Synonym Symbols
7B4; CD144
NCBI Protein Information
cadherin-5; 7B4 antigen; VE-cadherin; cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin; cadherin 5, type 2, VE-cadherin (vascular epithelium)
UniProt Protein Name
Cadherin-5
UniProt Gene Name
CDH5
UniProt Synonym Gene Names
VE-cadherin
UniProt Entry Name
CADH5_HUMAN
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Product Notes
The Canine CDH5 cdh5 (Catalog #AAA16736) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA16736 ELISA Kit recognizes Canine CDH5. It is sometimes possible for the material contained within the vial of "Cadherin 5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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