Application Data
PCTK3 recombinant protein
PCTK3 (CDK18)/CyclinY, Active
Gene Names
CDK18; PCTK3; PCTAIRE; PCTAIRE3
Applications
Western Blot
Synonyms
PCTK3; N/A; PCTK3 (CDK18)/CyclinY, Active; PCTK3 recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
PCTK3 (CDK18) (Full length) and Cyclin Y (2-end)
Sequence Length
3053
Applicable Applications for PCTK3 recombinant protein
WB (Western Blot), Kinase Assay
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
Application Data
Application Data
Application Data
Related Product Information for PCTK3 recombinant protein
Description: Full-length recombinant human PCTK3 (CDK18) and Cyclin Y (2-end) were co-expressed by baculovirus in Sf9 insect cells using N-terminal GST tags.
Scientific Background: PCTK3 or PCTAIRE protein kinase 3 is a member of the cdc2/cdkx subfamily of the ser/thr family of protein kinases. PCTK3 is thought to play a role in terminally differentiated neurons and it contains an N-terminal extension relative to CDC2 (1). PCTK3 is expressed in a variety of human cell lines and tissues (2). Elevation in the protein levels of PCTAIRE 3 in the temporal cortex of Alzheimer's disease relative to control brains, have been observed. It is proposed that PCTAIRE 3 is a paired helical filaments-associated kinase that modulates tau phosphorylation.
Scientific Background: PCTK3 or PCTAIRE protein kinase 3 is a member of the cdc2/cdkx subfamily of the ser/thr family of protein kinases. PCTK3 is thought to play a role in terminally differentiated neurons and it contains an N-terminal extension relative to CDC2 (1). PCTK3 is expressed in a variety of human cell lines and tissues (2). Elevation in the protein levels of PCTAIRE 3 in the temporal cortex of Alzheimer's disease relative to control brains, have been observed. It is proposed that PCTAIRE 3 is a paired helical filaments-associated kinase that modulates tau phosphorylation.
Product Categories/Family for PCTK3 recombinant protein
References
1. Meyerson, M.et.al: A family of human cdc2-related protein kinases. EMBO J. 11: 2909-2917, 1992. 2. Okuda, T.et.al: PCTAIRE-1 and PCTAIRE-3, two members of a novel cdc2/CDC28-related protein kinase gene family. Oncogene 7: 2249-2258, 1992.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
~76 kDa; ~64 kDa
NCBI Official Full Name
Homo sapiens PCTAIRE protein kinase 3, mRNA
NCBI Official Synonym Full Names
cyclin dependent kinase 18
NCBI Official Symbol
CDK18
NCBI Official Synonym Symbols
PCTK3; PCTAIRE; PCTAIRE3
NCBI Protein Information
cyclin-dependent kinase 18
UniProt Protein Name
Cyclin-dependent kinase 18
UniProt Gene Name
CDK18
UniProt Synonym Gene Names
PCTAIRE3; PCTK3
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Product Notes
The PCTK3 cdk18 (Catalog #AAA73041) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is PCTK3 (CDK18) (Full length) and Cyclin Y (2-end). AAA Biotech's PCTK3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Kinase Assay. Researchers should empirically determine the suitability of the PCTK3 cdk18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCTK3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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