Application Data
(Fig-1: Induction of NF-kB activity by PMA in FOXP3 Leeporter Jurkat T cells.)
FOXP3 Leeporter Luciferase Reporter Cell Line | FOXP3 cell line
FOXP3 Leeporter Luciferase Reporter-Jurkat Cell Line
Applications
Functional Assay
Synonyms
FOXP3 Leeporter Luciferase Reporter; N/A; FOXP3 Leeporter Luciferase Reporter-Jurkat Cell Line; Foxp3; Foxp3 reporter; Foxp3 luciferase reporter; Foxp3 cell line; Foxp3 reporter cell line; cell line; stable cell line; reporter cell line; luciferase; luciferase reporter cell line; leeporter; leeporter(TM); luciferase reporter; luciferase cell line; jurkat cell line; luciferase assay; FOXP3 cell line
Form/Format
Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Applicable Applications for FOXP3 cell line
FA (Functional Assay)
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Immediately upon receipt, store in liquid nitrogen.
Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Shipping Note: Product is available for shipment in the United States, Canada and European countries. Please inquire for shipment to other countries.
Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Shipping Note: Product is available for shipment in the United States, Canada and European countries. Please inquire for shipment to other countries.
Application Data
(Fig-1: Induction of NF-kB activity by PMA in FOXP3 Leeporter Jurkat T cells.)
Application Data
(Fig-1: Induction of NF-kB activity by PMA in FOXP3 Leeporter Jurkat T cells.)
Related Product Information for FOXP3 cell line
Description: The FOXP3 Leeporter™ Luciferase Reporter cell line is a stably transfected Jurkat T cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the Forkhead box P3 (FOXP3) promoter. As a member of the forkhead transcription factor family, FOXP3 is a key transcription factor that functions in the development and function of regulatory Tcells. Functional activity of the cell line has been validated by phorbol 12-myristate 13-acetate (PMA) in the presence of ionomycin
Culture Conditions:
Cells should be grown at 37oC with 5% CO2 using RPMI medium supplemented with 10% heat-inactivated FBS, 1 mM sodium pyruvate, 10 mM HEPES and 1% Pen/Strep plus 3 ug/ml of Puromycin (Note: Puromycin can be omitted during the reporter cell assays).
It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37°C water-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in 37°C-CO2 incubator.
Monitor the cell viability by counting cells daily for 1~3 days until cells completely recover viability as cells are doubling daily. Once cells are over 90% confluent, harvest cells by centrifugation and passage cells. At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence.
To passage the cells, transfer cells to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cell suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly. To achieve satisfactory results, cells should not be passaged over 16 times
Functional validation:
A Response of FOXP3 Leeporter™ – Jurkat T cells to phorbol 12-myristate 13-acetate (PMA)/ Ionomycin
1. Harvest FOXP3 Leeporter™ – Jurkat T cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium at 2.5 x 105 cells/well.
2. Right after plating cells, stimulate cells with various concentrations of PMA in the presence of 0.2 uM ionomycin and incubate cells at 37°C in a CO2 incubator for up to 24 hours.
3. Equilibrate the plate to room temperature for 10 minutes.
4. Add 50 ul of luciferase assay reagent (Cat # MBS668917 ; Refer to the reagent datasheet for the detailed luciferase assay protocol) per well
5. Read the plate in 1-5 minutes to measure luminescence using a microplate luminometer.
Culture Conditions:
Cells should be grown at 37oC with 5% CO2 using RPMI medium supplemented with 10% heat-inactivated FBS, 1 mM sodium pyruvate, 10 mM HEPES and 1% Pen/Strep plus 3 ug/ml of Puromycin (Note: Puromycin can be omitted during the reporter cell assays).
It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37°C water-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in 37°C-CO2 incubator.
Monitor the cell viability by counting cells daily for 1~3 days until cells completely recover viability as cells are doubling daily. Once cells are over 90% confluent, harvest cells by centrifugation and passage cells. At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence.
To passage the cells, transfer cells to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cell suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly. To achieve satisfactory results, cells should not be passaged over 16 times
Functional validation:
A Response of FOXP3 Leeporter™ – Jurkat T cells to phorbol 12-myristate 13-acetate (PMA)/ Ionomycin
1. Harvest FOXP3 Leeporter™ – Jurkat T cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium at 2.5 x 105 cells/well.
2. Right after plating cells, stimulate cells with various concentrations of PMA in the presence of 0.2 uM ionomycin and incubate cells at 37°C in a CO2 incubator for up to 24 hours.
3. Equilibrate the plate to room temperature for 10 minutes.
4. Add 50 ul of luciferase assay reagent (Cat # MBS668917 ; Refer to the reagent datasheet for the detailed luciferase assay protocol) per well
5. Read the plate in 1-5 minutes to measure luminescence using a microplate luminometer.
Product Categories/Family for FOXP3 cell line
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Product Notes
The FOXP3 (Catalog #AAA78447) is a Cell Line and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's FOXP3 Leeporter Luciferase Reporter can be used in a range of immunoassay formats including, but not limited to, FA (Functional Assay). Researchers should empirically determine the suitability of the FOXP3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXP3 Leeporter Luciferase Reporter, Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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