Mouse Complement Factor D ELISA Kit | CFD elisa kit
Mouse Complement Factor D ELISA Kit
Gene Names
CFD; DF; ADN; PFD; ADIPSIN
Reactivity
Mouse
Synonyms
Complement Factor D; N/A; Mouse Complement Factor D ELISA Kit; CFD/ADN/Adipsin/DF/PFD/Factor D/Properdin Factor D Esterase; CFD elisa kit
Reactivity
Mouse
Specificity
Specifically recognize CFD, no obvious cross reaction with other analogues
Sequence Length
253
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
46.875-3000pg/ml
Sensitivity
28.125pg/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for CFD elisa kit
Background: Complement factor D (CFD) is also known as Adipsin, C3 convertase activator and Properdin factor D (PFD), which contains one peptidase S1 domain and belongs to the peptidase S1 family. CFD cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. CFD is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. Defects in CFD are the cause of complement factor D deficiency which predisposes to invasive meningococcal disease.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti CFD antibody was precoated onto the 96-well plate. The biotin conjugated anti CFD antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with CFD conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of CFD in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti CFD antibody was precoated onto the 96-well plate. The biotin conjugated anti CFD antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with CFD conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of CFD in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,033 Da
NCBI Official Full Name
complement factor D isoform 1 preproprotein
NCBI Official Synonym Full Names
complement factor D (adipsin)
NCBI Official Symbol
CFD
NCBI Official Synonym Symbols
DF; ADN; PFD; ADIPSIN
NCBI Protein Information
complement factor D
UniProt Protein Name
Complement factor D
UniProt Gene Name
CFD
UniProt Synonym Gene Names
DF; PFD
UniProt Entry Name
CFAD_HUMAN
