Mouse Complement Factor H ELISA Kit | CFH elisa kit

Mouse Complement Factor H ELISA Kit

Cat. Num. AAA263110

• Gene Names: Cfh; NOM; Sas1; Mud-1; Sas-1
• Reactivity: Mouse
• Synonyms: Complement Factor H; N/A; Mouse Complement Factor H ELISA Kit; HF; HF1; HF2; Complement factor H; H factor 1; CFH elisa kit

• Reactivity: Mouse
• Specificity: The Mouse Complement Factor H ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse Complement Factor H proteins within the range of 780 pg/ml - 50000 pg/ml.
• Sequence Length: 1252
• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <20 pg/ml

• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Standard Curve (Sample)

Related Product Information for CFH elisa kit

Principle of the Assay: The Mouse Complement Factor H ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Complement Factor H in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse Complement Factor H coated on a 96-well plate. Standards and samples are pipetted into the wells and Complement Factor H present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Complement Factor H antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Complement Factor H bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternativecomplement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.

NCBI and Uniprot Product Information

• NCBI GI #: 109627652
• NCBI GeneID: 12628
• NCBI Accession #: NP_034018.2
• NCBI GenBank Nucleotide #: NM_009888.3
• UniProt Accession #: P06909; Q6NZK3
• Molecular Weight: 139,138 Da
• NCBI Official Full Name: complement factor H
• NCBI Official Synonym Full Names: complement component factor h
• NCBI Official Symbol: Cfh
• NCBI Official Synonym Symbols: NOM; Sas1; Mud-1; Sas-1
• NCBI Protein Information: complement factor H
• UniProt Protein Name: Complement factor H
• UniProt Gene Name: Cfh
• UniProt Synonym Gene Names: Hf1
• UniProt Entry Name: CFAH_MOUSE