Porcine Cystic Fibrosis Transmembrane Conductance Regulator ELISA Kit | CFTR elisa kit

Porcine Cystic Fibrosis Transmembrane Conductance Regulator ELISA Kit

Cat. Num. AAA88505

• Gene Names: CFTR; CF; MRP7; ABC35; ABCC7; CFTR/MRP; TNR-CFTR; dJ760C5.1
• Reactivity: Porcine
• Synonyms: Cystic Fibrosis Transmembrane Conductance Regulator; N/A; Porcine Cystic Fibrosis Transmembrane Conductance Regulator ELISA Kit; CFTR elisa kit

• Reactivity: Porcine
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.

• Intended Uses: This CFTR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine CFTR. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for CFTR elisa kit

Principle of the Assay: CFTR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CFTR antibody and an CFTR-HRP conjugate. The assay sample and buffer are incubated together with CFTR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CFTR concentration since CFTR from samples and CFTR-HRP conjugate compete for the anti-CFTR antibody binding site. Since the number of sites is limited, as more sites are occupied by CFTR from the sample, fewer sites are left to bind CFTR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CFTR concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CFTR elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 180286
• NCBI GeneID: 1080
• Molecular Weight: 69,230 Da
• NCBI Official Full Name: CFTR, partial
• NCBI Official Synonym Full Names: cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
• NCBI Official Symbol: CFTR
• NCBI Official Synonym Symbols: CF; MRP7; ABC35; ABCC7; CFTR/MRP; TNR-CFTR; dJ760C5.1
• NCBI Protein Information: cystic fibrosis transmembrane conductance regulator; cAMP-dependent chloride channel; channel conductance-controlling ATPase
• UniProt Protein Name: Cystic fibrosis transmembrane conductance regulator
• UniProt Gene Name: CFTR
• UniProt Synonym Gene Names: ABCC7; CFTR
• UniProt Entry Name: CFTR_HUMAN