Rat Chemerin ELISA Kit | CHEM elisa kit
Rat Chemerin ELISA Kit
Reactivity
Rat
Synonyms
Chemerin; N/A; Rat Chemerin ELISA Kit; RARRES2/TIG-2/chemerin/CHEM/RARRES2/RAR-responsive protein TIG2/retinoic acid receptor responder (tazarotene induced) 2/retinoic acid receptor responder protein 2/Tazarotene-induced gene 2 protein; CHEM elisa kit
Reactivity
Rat
Specificity
Specifically recognize CHEM, no obvious cross reaction with other analogues
Sequence Length
162
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
31.25-2000pg/ml
Sensitivity
18.75pg/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Related Product Information for CHEM elisa kit
Background: Chemerin is a 16KDa mature chemoattractant composed of 163 amino acids. It is involved in adipocyte differentiation and glucose transport through autocrine action, and can also mediate inflammatory response through paracrine and endocrine action. Chemerin is involved in immune response, inflammatory response and glucose and lipid metabolism, and shows both pro-inflammatory and anti-inflammatory effects. The expression of Chemerin is up-regulated by inflammatory cytokines (TNF-? and IL-6), and can promote angiogenesis.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti CHEM antibody was pre-coated onto the 96-well plate. The biotin conjugated anti CHEM antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with CHEM conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of CHEM in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti CHEM antibody was pre-coated onto the 96-well plate. The biotin conjugated anti CHEM antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with CHEM conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of CHEM in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
18,357 Da
NCBI Official Full Name
chemerin
UniProt Protein Name
Chemerin
UniProt Gene Name
RARRES2
UniProt Entry Name
B9VR25_BOVIN