Chicken Brain Natriuretic Peptide (BNP) ELISA Kit | BNP elisa kit
Chicken Brain Natriuretic Peptide (BNP) ELISA Kit
Reactivity
Chicken
Synonyms
Brain Natriuretic Peptide (BNP); N/A; Chicken Brain Natriuretic Peptide (BNP) ELISA Kit; BNP elisa kit
Reactivity
Chicken
Specificity
This assay has high sensitivity and excellent specificity for detection of BNP. No significant cross-reactivity or interference between BNP and analogues was observed.
Assay Type
Competitive Inhibition
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range
12.35-1,000pg/mL
Sensitivity
4.64pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BNP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BNP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all the reagents should be kept according to the label on each vial. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C while others should be at 4 degree C.
Standard Curve (Sample)
Related Product Information for BNP elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of BNP in gallus serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to BNP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled BNP and unlabeled BNP (Standards or samples) with the pre-coated antibody specific to BNP. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of BNP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of BNP in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to BNP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled BNP and unlabeled BNP (Standards or samples) with the pre-coated antibody specific to BNP. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of BNP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of BNP in the sample.
