Chicken Gallinacin-11 ELISA Kit | AvBD11 elisa kit

Chicken Gallinacin-11 ELISA Kit

Cat. Num. AAA247548

• Gene Names: AvBD11; GAL11; VMOII; GAL 11; VMO-II; gal-11
• Reactivity: Chicken
• Synonyms: Gallinacin-11; N/A; Chicken Gallinacin-11 ELISA Kit; AvBD11 elisa kit

• Reactivity: Chicken
• Specificity: This assay has high sensitivity and excellent specificity for detection of GAL11. No significant cross-reactivity or interference between GAL11 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GAL11 and all the analogues, therefore, cross reaction may still exist in some cases.
• Sequence Length: 104
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL

• Reactivity Note: No significant cross-reactivity or interference between this antigen and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection, therefore, cross reaction may still exist in some cases.
• Preparation and Storage: Store at 2-8 degree C.; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 127% within the expiration date under appropriate storage condition.

Standard Curve (Sample)

Related Product Information for AvBD11 elisa kit

Principle of the Assay: GAL11 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GAL11 antibody and an GAL11-HRP conjugate. The assay sample and buffer are incubated together with GAL11-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAL11 concentration since GAL11 from samples and GAL11-HRP conjugate compete for the anti-GAL11 antibody binding site. Since the number of sites is limited, as more sites are occupied by GAL11 from the sample, fewer sites are left to bind GAL11-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAL11 concentration in each sample is interpolated from this standard curve.

Intended Uses: This GAL11 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken GAL11. This ELISA kit for research use only, not for therapeutic or test applications!

NCBI and Uniprot Product Information

• NCBI GI #: 49169808
• NCBI GeneID: 414876
• NCBI Accession #: NP_001001779.1
• NCBI GenBank Nucleotide #: NM_001001779.1
• UniProt Accession #: Q6IV20; Q09MT4; Q9PS49; A0A1W6
• Molecular Weight: 11,650 Da
• NCBI Official Full Name: gallinacin-11
• NCBI Official Synonym Full Names: avian beta-defensin 11
• NCBI Official Symbol: AvBD11
• NCBI Official Synonym Symbols: GAL11; VMOII; GAL 11; VMO-II; gal-11
• NCBI Protein Information: gallinacin-11
• UniProt Protein Name: Gallinacin-11
• UniProt Gene Name: GAL11
• UniProt Synonym Gene Names: Gal-11; VMO-II; VMOII