Granulocyte-Colony Stimulating Factor Active Protein | G CSF active protein

Recombinant Human Granulocyte-Colony Stimulating Factor, CHO

Cat. Num. AAA38160

• Gene Names: CSF3; GCSF; CSF3OS; C17orf33
• Purity: Greater than 97.0% as determined by: a) Analysis by RP-HPLC. b) Analysis by SDS-PAGE.
• Synonyms: Granulocyte-Colony Stimulating Factor; N/A; Recombinant Human Granulocyte-Colony Stimulating Factor, CHO; G CSF Human, CHO; Granulocyte-Colony Stimulating Factor Human Recombinant, CHO; CSF-3; MGI-1G; GM-CSF beta; Pluripoietin; Filgrastim; Lenograstim; G-CSF; MGC45931; GCSF; G CSF CHO; G CSF active protein

• Host: Chinese Hamster Ovarian Cells (CHO)
• Purity/Purification: Greater than 97.0% as determined by: a) Analysis by RP-HPLC. b) Analysis by SDS-PAGE.
• Form/Format: G-CSF was lyophilized from a concentrated (1mg/ml) Phosphate- Buffered Saline, pH 7.4.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP
• Sequence Length: 207

• Solubility: It is recommended to reconstitute the lyophilized Granulocyte Colony Stimulating Factor in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Content: GCSF quantitation was carried out by two independent methods:1) UV spectroscopy at 280 nm using the absorbency value of 0.815 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2) Analysis by RP-HPLC, using a calibrated solution of Granulocyte Colony Stimulating Factor as a Reference Standard.
• Biological Activity: The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is < 0.07 ng/ml, corresponding to a Specific Activity of 1.27 x 108 IU/mg.
• Preparation and Storage: Lyophilized Granulocyte Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution G-CSF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for G CSF active protein

Description: Granulocyte Colony Stimulating Factor Human Recombinant produced in CHO cells is a single, glycosylated, polypeptide chain containing 174 amino acids and having a molecular mass of approximately 18 kDa.G-CSF is purified by proprietary chromatographic techniques.

Introduction: Granulocyte Colony Stimulating Factor is a growth factor and/or cytokine produced by the endothelium, macrophages and a number of other immune cells. GCSF stimulates the bone marrow to produce granulocytes and also to stimulate the survival, proliferation, differentiation and function of neutrophil granulocyte progenator cells and mature neutrophils.

Product Categories/Family for G CSF active protein

Cytokines and Growth Factors; Growth Factors; Colony Stimulating Factor

NCBI and Uniprot Product Information

• NCBI GI #: 4503079
• NCBI GeneID: 1440
• NCBI Accession #: NP_000750.1
• NCBI GenBank Nucleotide #: NM_000759.3
• UniProt Accession #: P09919; A8MXR7
• Molecular Weight: 18,434 Da
• NCBI Official Full Name: granulocyte colony-stimulating factor isoform a
• NCBI Official Synonym Full Names: colony stimulating factor 3 (granulocyte)
• NCBI Official Symbol: CSF3
• NCBI Official Synonym Symbols: GCSF; CSF3OS; C17orf33
• NCBI Protein Information: granulocyte colony-stimulating factor; filgrastim; granulocyte-colony stimulating factor; lenograstim; pluripoietin
• UniProt Protein Name: Granulocyte colony-stimulating factor
• UniProt Gene Name: CSF3
• UniProt Synonym Gene Names: C17orf33; GCSF; G-CSF
• UniProt Entry Name: CSF3_HUMAN