Hepatocyte Growth Factor Active Protein | Hgf active protein

Mouse Hepatocyte Growth Factor Recombinant

Cat. Num. AAA76400

• Gene Names: Hgf; SF; NK1; NK2; HGF/SF; SF/HGF; C230052L06Rik
• Purity: >97%, as determined by SDS-PAGE and HPLC
• Synonyms: Hepatocyte Growth Factor; N/A; Mouse Hepatocyte Growth Factor Recombinant; Hgf active protein

• Host: Chinese Hamster Ovary cell line (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
• Purity/Purification: >97%, as determined by SDS-PAGE and HPLC
• Form/Format: Mouse HGF was lyophilized from a 0.2 um filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween80, 5 mM Glutamic acid, pH 4.5.
• Sequence: MMWGTKLLPV LLLQHVLLHL LLLHVAIPYA EGQKKRRNTL HEFKKSAKTT 50 LTKEDPLLKI KTKKVNSADE CANRCIRNRG FTFTCKAFVF DKSRKRCYWY 100 PFNSMSSGVK KGFGHEFDLY ENKDYIRNCI IGKGGSYKGT VSITKSGIKC 150 QPWNSMIPHE HSFLPSSYRG KDLQENYCRN PRGEEGGPWC FTSNPEVRYE 200 VCDIPQCSEV ECMTCNGESY RGPMDHTESG KTCQRWDQQT PHRHKFLPER 250 YPDKGFDDNY CRNPDGKPRP WCYTLDPDTP WEYCAIKTCA HSAVNETDVP 300 METTECIQGQ GEGYRGTSNT IWNGIPCQRW DSQYPHKHDI TPENFKCKDL 350 RENYCRNPDG AESPWCFTTD PNIRVGYCSQ IPKCDVSSGQ DCYRGNGKNY 400 MGNLSKTRSG LTCSMWDKNM EDLHRHIFWE PDASKLNKNY CRNPDDDAHG 450 PWCYTGNPLI PWDYCPISRC EGDTTPTIVN LDHPVISCAK TKQLR>VVNGI 500 PTQTTVGWMV SLKYRNKHIC GGSLIKESWV LTARQCFPAR NKDLKDYEAW 550 LGIHDVHERG EEKRKQILNI SQLVYGPEGS DLVLLKLARP AILDNFVSTI 600 DLPSYGCTIP EKTTCSIYGW GYTGLINADG LLRVAHLYIM GNEKCSQHHQ 650 GKVTLNESEL CAGAEKIGSG PCEGDYGGPL ICEQHKMRMV LGVIVPGRGC 700 AIPNRPGIFV RVAYYAKWIH KVILTYKL

• Source: Optimized DNA sequence encoding Mouse Hepatocyte Growth Factor mature chain was expressed in Chinese Hamster Ovary cell line.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Biological Activity: The ED(50) was determined by the dose-dependent stimulation of the proliferation ofmurine BNL-CL2cell li was found to be in the range of 20.0-40.0 ng/ml.
• Method: 2.4. In Vitro Hepatic DifferentiationPrior to starting the hepatic differentiation, medium'>MSCs at passage 5 were maintained in the regular medium'>MSC culture medium until at 80-90% confluence.; The hepatic differentiation was elicited in the differentiation-inducing medium, which consisted of MEM supplemented with 10% FBS, 10?ng/mL hepatocyte growth factor (HGF), 10?ng/mL basic fibroblast growth factor (bFGF) and 10?ng/mL oncostatin M (&system).; The medium was changed every 3 days, and the cells were cultured for 8 days.; In vitro differentiation of hepatocyte-like cells from undifferentiated iPS cells; >Stage 3: Differentiation of Premature Hepatocyte-Like Cells (5-11).; At day 5, the medium was replaced by Knockout DMEM supplemented with 1% KSR, 1% nonessential amino acids, 1% l-glutamic acid, 1% dimethyl sulfoxide (DMSO), and 100 ng/ml hepatocyte growth factor (HGF) to induce premature hepatocyte-like cells.; The medium was replaced every two days.; Cell CultureNPCs were prepared from striatum of embryonic day 17 to 18 (E17-18) old rats and the cells were plated in dishes (5×106 cells per 10 cm dish,,) and cultivated in a medium'>serum-free medium'>DMEM/F12 medium in the presence of B27 supplement and 20 ng/ml epidermal growth factor (EGF) at +37 degree C in 5% CO2; Cell culture and hepatic cell differentiationHuh7 and Huh7.5 cells were provided by Jane C. Moores (The Reagent of the University of California, Oakland, CA).The cells were maintained in medium'>Dulbecco's medium'>modified medium'>Eagle's medium (DMEM;,,) containing 10% FBS and 1% penicillin/streptomycin in a humidified incubator at 37 degree C with 5% CO2.; For hepatic differentiation, passages 3-7 of AT-hMSCs were plated at 1×104 cells/cm2 and incubated overnight.; The cells were then washed with phosphate-buffered saline (PBS) and incubated in basal medium [60% DMEM-low glucose, 40% MCDB201, and 1% penicillin/streptomycin] with 20 ng/ml EGF and 10 ng/ml bFGF for 2 days.; After incubation, hepatogenic cytokines and growth factors were sequentially added as follows: days 0-7 (step 1), basal medium with 20 ng/ml HGF and 10 ng/ml bFGF; and days 7-21 (step 2), basal medium containing 20 ng/ml oncostatin M, 1 umol/L dexamethasone, and 50 mg/ml insulin-transferrin-selenium (ITS) premix.The media were changed every 3 days.Differentiation of hESCs into dopaminergic neurons, osteoblasts, and hepatocytesDerivation of hepatocytes from hESCs was carried out as previously described (uM dexamethasone for 5 days.
• Molecular Function: Growth-factor, Serine-protease-homolog
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at-20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Application Data

(Conditioned media from BMPC regulates miR-155 expression and fibrogenic response in mouse cardiac fibroblasts in vitro.Neutralizing antibodies against HGF (HGF-Ab) reversed this effect as compared to IgG treated cells.)

Application Data

(Met knockdown reduces BxPC-3 and ASPC-1 cell survival.BxPC-3 or ASPC-1 cells infected with recombinant lentivirus expressing Met knockdown shRNAs (2 or 5) or a non targeting shRNA were treated without or with HGF and examined by Western analysis for pMet (Y1234/1235), Met, pErk1/2, Erk1/2, pAKT, AKT and ?-actin levels (n?=?3).)

Product Categories/Family for Hgf active protein

Cytokines; Mouse reagents; Recombinant Mouse Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 575501559
• NCBI GeneID: 15234
• NCBI Accession #: NP_001276387.1
• NCBI GenBank Nucleotide #: NM_001289458.1
• UniProt Accession #: Q08048; P08581 MET_HUMAN; P46414
• Molecular Weight: 24,308 Da
• NCBI Official Full Name: hepatocyte growth factor isoform 1 preproprotein
• NCBI Official Synonym Full Names: hepatocyte growth factor
• NCBI Official Symbol: Hgf
• NCBI Official Synonym Symbols: SF; NK1; NK2; HGF/SF; SF/HGF; C230052L06Rik
• NCBI Protein Information: hepatocyte growth factor
• UniProt Protein Name: Hepatocyte growth factor
• UniProt Gene Name: Hgf
• UniProt Synonym Gene Names: SF
• UniProt Entry Name: HGF_MOUSE