Interferon-beta 1b Active Protein | IFNB1 active protein

Human Interferon-beta 1b Recombinant

Cat. Num. AAA76387

• Gene Names: IFNB1; IFB; IFF; IFNB
• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Interferon-beta 1b; N/A; Human Interferon-beta 1b Recombinant; IFNB1 active protein

• Host: Chinese Hamster Ovary cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Interferon betabwas lyophilized from a 0.2 um filtered solution containingmM NaOAc pH.5.
• Sequence: MTNKCLLQIA LLLCFSTTAL SMSYNLLGFL QRSSNFQCQK LLWQLNGRLE YCLKDRMNFD IPEEIKQLQQ FQKEDAALTI YEMLQNIFAI FRQDSSSTGW NETIVENLLA NVYHQINHLK TVLEEKLEKE DFTRGKLMSS LHLKRYYGRI LHYLKAKEYS HCAWTIVRVE ILRNFYFINR LTGYLRN

• Source: Optimized DNA sequence encoding Human beta Interferon 1b mature chain was expressed in Chinese Hamster Ovary cells.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Biological Activity: Theactivity was determined by the cytopathic inhibition assay of human WISH cells infected with the ECMV virus, and determined to be 1x107 IU/mg.
• Method: Islet isolation, culture, and exposure to different beta-cell inhibitory substancesFollowing the initial culture period, islets were cultured for an additional 6-24 hours in CMRL 1066 containing antibiotics, 2 mM glutamine and one of the following supplements: 0.5 mM sodium palmitate solubilized in 0.5% (weight/volume) fatty acid and lipopolysaccaride free bovine serum albumin (BSA) ; recombinant human Interleukin 1beta (IL-1beta (50 units/ml) and Interferon-gamma (IFN-gamma) (1,000 units/ml) ; 100 mM hydrogen peroxide; 2 mM DETA/NO or 10 mM streptozotocin (STZ).; To some of the groups 10 uM of Imatinib was added at different time points prior to the addition of test substances given above.; To controls equal amounts of vehicle (DMSO) were supplemented.Western blotting for NF-kappaB, IkappaB-alpha and Smad7Interferon gamma (IFN-gamma) 50 ul (100 ng/ml) was added to each dish in the experimental studies.; The cytoplasmic and nuclear extracts were washed with ice-cold PBS and lysed in a 0.5 ml/well lysis buffer (150 mmol/l NaCl, 20 mmol/l Tris, pH 7.5, 0.1% Triton X-100, 1 mmol/l phenylmethylsulfonyl fluoride [PMSF] and 10 ug/ml aprotonin) as modified from the reports of Kim et al.and Moon et al.CIK induction and intravenous infusionVenous blood was collected prior to chemotherapy or at least one month following chemotherapy.; For the offspring (HLA haploidentical donors), the routine blood tests, and liver and kidney function tests were normal and negative for hepatitis A virus-IgM, hepatitis B surface antigen antibody, hepatitis B e--hepatitis B core-hepatitis C virus (HCV)-HCV-IgG, Syphilis-and human immunodeficiency virus-.As reported in our previous study (2 atmosphere.; On the day of culture, 1,000 U/ml human recombinant interferon-gamma, 500 U/ml recombinant human interleukin (rhIL)-1alpha and 1,000 U/ml rhIL-2 (Quangang Pharmaceutical Co.,,,) were added.; Four days later, 1,000 U/ml rhIL-2 was added and the cells were transferred into a GT-T610 culture bag.; Cell growth was observed every other day and cells were stained with 0.4% trypan blue and the viable cells were counted.Following 18 days of culture, cells were infused once daily (>1×109 cells; viability rate, >95%).; A cycle of treatment comprised of between three and five infusions and >5×109 cells were infused per cycle.; Prior to infusion, the surface immunophenotype of CIKs was determined by a standard fluorescence cytometry labeling protocol using fluorochrome-conjugated monoclonal mouse anti-human antibodies against CD3, CD8, CD4, CD45, CD19 and CD16CD56.Assays for the detection of bacteria, mildew and endotoxin were performed.; Briefly, the presence of bacteria and mildew was investigated by incubating cultured CIKs on agar at 37 degree C, with subsequent inspection for the growth of microbial contaminants.; Endotoxin levels were determined using a chromogenic endotoxin assay kit (Crab Reagent Manufactory,,,,), according to the manufacturer's instructions.; Only sterile and endotoxin-free preparations were used clinically.Invasion assayCell migration through Matrigel-coated filters was measured using Transwell chambers with 8 um-pore polycarbonate filters coated with Matrigel matrix as previously described (4 cells/well in the upper compartment of each invasion chamber and incubated for 24 h in the absence or presence of 100 U/ml interferon (IFN)-gamma, 10 ng/ml interleukin (IL)1-alpha or 25 ng/ml tumor necrosis factor (TNF)-alpha, plus extract of Cnidiihizoma (0.1-5 mg/ml) or 1400W (0.5 mM).; Non-migrating cells on the upper surface of the membrane were gently scrubbed with a cotton swab, and the invading cells on the lower surface were fixed with 100% methanol and stained with hematoxylin and eosin Y solution (RICCA Chemical Company, Charlotte, NC, USA).; The number of cells was counted under a microscope at a magnification of ×100.
• Molecular Function: Cytokine
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least years from date of receipt at-20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Application Data

((A) Nitrite production by HT-29 cells following 48 h of treatment with cytokines.Effect of 0–50 ng/ml TNF-? on nitrite production induced by 100 U/ml IFN-? and 10 ng/ml IL-1? in HT-29 cells following 48 h of treatment.)

Application Data

(Replicative control of HCV in JFH-1/Huh7.5.1 system with conditioned media (CM) from pU/UC-transfected pDC-GEN2.2 cells.E) Recombinant Type III Interferons in the absence of CM at the same concentrations as found in the CM (IL-28A/IFN?2: 1500 pg/mL; IL-28B/IFN?3: 10 pg/mL; IL-29/IFN?1: 500 pg/mL) were added to JFH-1 infected Huh7.5.1 cells.)

Product Categories/Family for IFNB1 active protein

Interferon-IL10 family; Recombinant Human Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 4504603
• NCBI GeneID: 3456
• NCBI Accession #: NP_002167.1
• NCBI GenBank Nucleotide #: NM_002176.2
• UniProt Accession #: P01574
• Molecular Weight: 22,294 Da
• NCBI Official Full Name: interferon beta
• NCBI Official Synonym Full Names: interferon, beta 1, fibroblast
• NCBI Official Symbol: IFNB1
• NCBI Official Synonym Symbols: IFB; IFF; IFNB
• NCBI Protein Information: interferon beta; IFN-beta; fibroblast interferon
• UniProt Protein Name: Interferon beta
• UniProt Gene Name: IFNB1
• UniProt Synonym Gene Names: IFB; IFNB; IFN-beta
• UniProt Entry Name: IFNB_HUMAN