SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
CHMP2A recombinant protein
CHMP2A, 1-222aa, Human, His tag, E Coli
Gene Names
CHMP2A; BC2; BC-2; VPS2; CHMP2; VPS2A
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
CHMP2A; N/A; CHMP2A, 1-222aa, Human, His tag, E Coli; Chromatin modifying protein 2A; BC-2; BC2; CHMP2; VPS2; VPS2A; Chromatin modifying protein 2A Charged multivesicular body protein 2a Vps2 1; hVps2 1; putative breast adenocarcinoma marker; Putative breast adenocarcinoma marker BC2; Vacuolar protein sorting associated protein 2 1.; CHMP2A recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.1 M NaCl 1mM DTT, and 30% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MDLLFGRRKT PEELLRQNQR ALNRAMRELD RERQKLETQE KKIIADIKKM AKQGQMDAVR IMAKDLVRTR RYVRKFVLMR ANIQAVSLKI QTLKSNNSMA QAMKGVTKAM GTMNRQLKLP QIQKIMMEFE RQAEIMDMKE EMMNDAIDDA MGDEEDEEES DAVVSQVLDE LGLSLTDELS NLPSTGGSLS VAAGGKKAEA AASALADADA DLEERLKNLR RD
Sequence Length
222
Applicable Applications for CHMP2A recombinant protein
SDS-PAGE
Domain
1-222aa
Tag
His-Tag
Preparation and Storage
Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for CHMP2A recombinant protein
CHMP2A, also known as chromatin modifying protein 2A, belongs to the SNF7 family and it is component of the ESCRT III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. Recombinant CHMP2A protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for CHMP2A recombinant protein
References
Lata S., et al. (2008) Science. 321 (5894):1354-7. ; Agromayor M., et al. (2006) J Biol Chem. 281 (32):23083-91.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27.2 kDa (242aa), confirmed by MALDI-TOF.
NCBI Official Full Name
charged multivesicular body protein 2a
NCBI Official Synonym Full Names
charged multivesicular body protein 2A
NCBI Official Symbol
CHMP2A
NCBI Official Synonym Symbols
BC2; BC-2; VPS2; CHMP2; VPS2A
NCBI Protein Information
charged multivesicular body protein 2a
UniProt Protein Name
Charged multivesicular body protein 2a
UniProt Gene Name
CHMP2A
UniProt Synonym Gene Names
BC2; CHMP2; CHMP2a; Vps2-1; hVps2-1
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Product Notes
The CHMP2A chmp2a (Catalog #AAA11771) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CHMP2A can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the CHMP2A chmp2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MDLLFGRRKT PEELLRQNQR ALNRAMRELD RERQKLETQE KKIIADIKKM AKQGQMDAVR IMAKDLVRTR RYVRKFVLMR ANIQAVSLKI QTLKSNNSMA QAMKGVTKAM GTMNRQLKLP QIQKIMMEFE RQAEIMDMKE EMMNDAIDDA MGDEEDEEES DAVVSQVLDE LGLSLTDELS NLPSTGGSLS VAAGGKKAEA AASALADADA DLEERLKNLR RD. It is sometimes possible for the material contained within the vial of "CHMP2A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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