Testing Data
(NGF contents in the skin.NGF contents in the skin of the affected and contralateral hindlimbs of wild-type and Y1472F-KI mice on day 50 post-inoculation and in the back skin at 24?h after wounding were measured by ELISA.)
beta Nerve Growth Factor Active Protein | Ngf active protein
Mouse beta Nerve Growth Factor Recombinant
Gene Names
Ngf; Ngfb
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
beta Nerve Growth Factor; N/A; Mouse beta Nerve Growth Factor Recombinant; Ngf active protein
Host
CHO cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant mouse NGF was lyophilized from a 0.2 um filtered solution inmM sodium acetate, pH.5.
Sequence
MSMLFYTLIT AFLIGVQAEP YTDSNVPEGD SVPEAHWTKL QHSLDTALRR ARSAPTAPIA ARVTGQTRNI TVDPRLFKKR RLHSPRVLFS TQPPPTSSDT LDLDFQAHGT IPFNRTHRSK RSSTHPVFHM GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL AEVNINNSVF RQYFFETKCR ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKATRR G
Source
Optimized DNA sequence encoding Mouse Nerve Growth Factor mature chain was expressed in CHO.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Biological Activity
The ED(50) was determined by the determined by its ability to stimulate chick E9 DRG neurite outgrowth was found to be in the range of1.0 ng/ml.
Molecular Function
Growth-factor, Metalloenzyme-inhibitor, Protease-inhibitor
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Preparation and Storage
The lyophilized protein is stable for at least years from date of receipt at-20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Testing Data
(NGF contents in the skin.NGF contents in the skin of the affected and contralateral hindlimbs of wild-type and Y1472F-KI mice on day 50 post-inoculation and in the back skin at 24?h after wounding were measured by ELISA.)
Testing Data
(NGF contents in the skin.NGF contents in the skin of the affected and contralateral hindlimbs of wild-type and Y1472F-KI mice on day 50 post-inoculation and in the back skin at 24?h after wounding were measured by ELISA.)
Product Categories/Family for Ngf active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,077 Da
NCBI Official Full Name
beta-nerve growth factor isoform B
NCBI Official Synonym Full Names
nerve growth factor
NCBI Official Symbol
Ngf
NCBI Official Synonym Symbols
Ngfb
NCBI Protein Information
beta-nerve growth factor; beta-NGF; nerve growth factor, beta
UniProt Protein Name
Beta-nerve growth factor
UniProt Gene Name
Ngf
UniProt Synonym Gene Names
Ngfb; Beta-NGF
UniProt Entry Name
NGF_MOUSE
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Product Notes
The Ngf ngf (Catalog #AAA333006) is an Active Protein produced from CHO cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.) and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MSMLFYTLIT AFLIGVQAEP YTDSNVPEGD SVPEAHWTKL QHSLDTALRR ARSAPTAPIA ARVTGQTRNI TVDPRLFKKR RLHSPRVLFS TQPPPTSSDT LDLDFQAHGT IPFNRTHRSK RSSTHPVFHM GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL AEVNINNSVF RQYFFETKCR ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKATRR G. It is sometimes possible for the material contained within the vial of "beta Nerve Growth Factor, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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