IL-6 receptor alpha Active Protein | IL-6 active protein
Human IL-6 receptor alpha, soluble
Gene Names
IL6R; IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1
Reactivity
Human
Purity
> 95% by SDS-PAGE & HPLC analyses
Synonyms
IL-6 receptor alpha; N/A; Human IL-6 receptor alpha, soluble; soluble IL-6 receptor alpha; B cell stimulatory factor-2; CD126; IL-6 active protein
Host
CHO cells
Reactivity
Human
Purity/Purification
> 95% by SDS-PAGE & HPLC analyses
Form/Format
Lyophilized
Sequence
LAPRRCPAQE VARGVLTSLP GDSVTLTCPG VEPEDNATVH WVLRKP AAGS HPSRWAGMGR RLLLRSVQLH DSGNYSCYRA GRPAGTVHLL V DVPPEEPQL SCFRKSPLSN VVCEWGPRST PSLTTKAVLL VRKFQNS PAE DFQEPCQYSQ ESQKFSCQLA VPEGDSSFYI VSMCVASSVG SK FSKTQTFQ GCGILQPDPP ANITVTAVAR NPRWLSVTWQ DPHSWNSS FY RLRFELRYRA ERSKTFTTWM VKDLQHHCVI HDAWSGLRHV VQL RAQEEFG QGEWSEWSPE AMGTPWTESR SPPAENEVST PMQALTTNK D DDNILFRDSA NATSLPVQD
Sequence Length
339
Endotoxin level
< 0.1 ng/ug of protein (<1EU/ug).
Biological Activity
Determined by its ability to intensify the IL-6 induced growth inhibition of mouse M1 cells. The expected ED50 is <= 5.0 ng/ml, in the presence of 20 ng/ml of rhIL-6.
Preparation and Storage
Stability:
The lyophilized protein is stable at room temperature for 1 month and at 4°C for 3 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.
Reconstitution:
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/m. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
AVOID REPEATED FREEZE AND THAW CYCLES!
The lyophilized protein is stable at room temperature for 1 month and at 4°C for 3 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.
Reconstitution:
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/m. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
AVOID REPEATED FREEZE AND THAW CYCLES!
Related Product Information for IL-6 active protein
IL-6 mediates its biological effects through the type I IL-6 receptor system that consists of two chains, IL-6Ralpha and gp130. While the IL-6Ralpha chain is the binding component specific to IL-6, the gp130 chain only transmits signals of IL-6 when bound to IL-6Ralpha. The gp130 can also transmit signals from LIF, OSM, CNTF, IL-11 and CT-1 in conjunction with other receptor subunits. The low-affinity binding site for IL-6 is composed of IL-6Ralpha alone. IL-6Ralpha is expressed in a wide range of cells, including T cells, fibroblasts and macrophages. Soluble IL-6Ralpha, which consists of only the extracellular domain of the IL-6Ralpha chain, acts as an agonist of IL-6 activity at low concentrations. Recombinant Human sIL-6 Receptor alpha is a 37.9 kDa glycoprotein corresponding to 339 amino acid residues of the extracellular domain of IL-6Ralpha. As a result of glycosylation, Recombinant Human sIL-6 Receptor alpha migrates with an apparent molecular mass of approximately 57-70 kDa by SDS-PAGE gel, under reducing conditions.
Product Categories/Family for IL-6 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57-70 kDa (reducing conditions)
NCBI Official Full Name
interleukin-6 receptor subunit alpha isoform 1
NCBI Official Synonym Full Names
interleukin 6 receptor
NCBI Official Symbol
IL6R
NCBI Official Synonym Symbols
IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1
NCBI Protein Information
interleukin-6 receptor subunit alpha
UniProt Protein Name
Interleukin-6 receptor subunit alpha
UniProt Gene Name
IL6R
UniProt Synonym Gene Names
IL-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; gp80
Similar Products
Product Notes
The IL-6 il6r (Catalog #AAA79325) is an Active Protein produced from CHO cells and is intended for research purposes only. The product is available for immediate purchase. The Human IL-6 receptor alpha, soluble reacts with Human and may cross-react with other species as described in the data sheet. The amino acid sequence is listed below: LAPRRCPAQE VARGVLTSLP GDSVTLTCPG VEPEDNATVH WVLRKP AAGS HPSRWAGMGR RLLLRSVQLH DSGNYSCYRA GRPAGTVHLL V DVPPEEPQL SCFRKSPLSN VVCEWGPRST PSLTTKAVLL VRKFQNS PAE DFQEPCQYSQ ESQKFSCQLA VPEGDSSFYI VSMCVASSVG SK FSKTQTFQ GCGILQPDPP ANITVTAVAR NPRWLSVTWQ DPHSWNSS FY RLRFELRYRA ERSKTFTTWM VKDLQHHCVI HDAWSGLRHV VQL RAQEEFG QGEWSEWSPE AMGTPWTESR SPPAENEVST PMQALTTNK D DDNILFRDSA NATSLPVQD. It is sometimes possible for the material contained within the vial of "IL-6 receptor alpha, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.