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Application Data
(TGF-? suppresses DC production but has no effect on DC activation.Bar graphs summarize the average production of IL-12, IL-6, and IL-10 from CD11cdnR (n?=?4) and wild-type (n?=?4) cultures.)
Interleukin-12 p40 Active Protein | Il12b active protein
Mouse Interleukin-12 p40 Recombinant
Gene Names
Il12b; p40; Il-12b; Il12p40; Il-12p40
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
Interleukin-12 p40; N/A; Mouse Interleukin-12 p40 Recombinant; Il12b active protein
Host
CHO cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant Interleukin-12 was lyophilized from a 0.2 um filtered PBS solution.
Sequence
MCPQKLTISW FAIVLLVSPL MAMWELEKDV YVVEVDWTPD APGETVNLTC DTPEEDDITW TSDQRHGVIG SGKTLTITVK EFLDAGQYTC HKGGETLSHS HLLLHKKENG IWSTEILKNF KNKTFLKCEA PNYSGRFTCS WLVQRNMDLK FNIKSSSSSP DSRAVTCGMA SLSAEKVTLD QRDYEKYSVS CQEDVTCPTA EETLPIELAL EARQQNKYEN YSTSFFIRDI IKPDPPKNLQ MKPLKNSQVE VSWEYPDSWS TPHSYFSLKF FVRIQRKKEK MKETEEGCNQ KGAFLVEKTS TEVQCKGGNV CVQAQDRYYN SSCSKWACVP CRVRS
Source
Optimized DNA sequence encoding mouse Interleukin-12 mature chain was over-expressed in CHo cells.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Biological Activity
The ED(50) determined by the dose-dependent stimulation of IFN-gamma production by murine splenocytes co-stimulated with IL-18 is <.1 ng/ml, corresponding to a specific activity of > x units/mg.
Method
Mouse T Cell Primary CulturesPeripheral lymph node cells were pooled from axillary, inguinal, brachial, and cervical lymph nodes (excluding mesenteric (mes)LN).
Where explicitly stated in the figure legends mesLN were collected.
After lymphoid organ disaggregation, cells were washed and resuspended in complete media (RPMI-1640, 5%FCS, 2 mM L-gln, 100 U/ml penicillin/streptomycin, and 2.5×10-5 M 2beta-ME.
Anti-CD3 (145-2C11, BD) at 0-1 ug/ml and anti-CD28 (37.51, BD) at 2 ug/ml mAb were coated on plates (1h, 37 degree C, in PBS) and washed twice.
In transient TCR stimulations, cells were moved from Ab-coated to fresh wells on d2.
aAPC were described previouslyFunctional assays with T cells of healthy donorscelltype'>T cell activation experiments using the system of celltype'>T cell stimulator cells were done as previously described 4/well) were co-cultured with 1×105 celltype'>T cells, and following 48 h of stimulation, cell culture supernatant was harvested for cytokine measurement and methyl-3[H]-thymidine was added to the cultures.Expression of membrane-bound anti-CD3 antibody fragments on the T cell stimulator cells was detected via DyLight-649-conjugated goat antibodies to mouse IgG-H+L specific.
Isolation of human T cells and monocytes and generation of immature and mature monocyte-derived DC was done as previously described 5 T cells/well were added and cultured for 48 h in the presence of soluble TIM-3 antibodies or isotype control antibodies (bothfinal concentration: 5 ug/ml) as previously described 5/well) were co-cultured with allogeneic immature or mature human DC at the indicated cell numbers for 5 days in presence of the indicated antibodies (final concentration 10 ug/ml).
To assess T cell proliferation methyl-3[H]-thymidine (final concentration: 0.025 mCi Elmer/New England Nuclear Corporation,,) was added for the last 18 hours of.<>Methyl-3[H]-thymidine uptake was measured as described
Where explicitly stated in the figure legends mesLN were collected.
After lymphoid organ disaggregation, cells were washed and resuspended in complete media (RPMI-1640, 5%FCS, 2 mM L-gln, 100 U/ml penicillin/streptomycin, and 2.5×10-5 M 2beta-ME.
Anti-CD3 (145-2C11, BD) at 0-1 ug/ml and anti-CD28 (37.51, BD) at 2 ug/ml mAb were coated on plates (1h, 37 degree C, in PBS) and washed twice.
In transient TCR stimulations, cells were moved from Ab-coated to fresh wells on d2.
aAPC were described previouslyFunctional assays with T cells of healthy donorscelltype'>T cell activation experiments using the system of celltype'>T cell stimulator cells were done as previously described 4/well) were co-cultured with 1×105 celltype'>T cells, and following 48 h of stimulation, cell culture supernatant was harvested for cytokine measurement and methyl-3[H]-thymidine was added to the cultures.Expression of membrane-bound anti-CD3 antibody fragments on the T cell stimulator cells was detected via DyLight-649-conjugated goat antibodies to mouse IgG-H+L specific.
Isolation of human T cells and monocytes and generation of immature and mature monocyte-derived DC was done as previously described 5 T cells/well were added and cultured for 48 h in the presence of soluble TIM-3 antibodies or isotype control antibodies (bothfinal concentration: 5 ug/ml) as previously described 5/well) were co-cultured with allogeneic immature or mature human DC at the indicated cell numbers for 5 days in presence of the indicated antibodies (final concentration 10 ug/ml).
To assess T cell proliferation methyl-3[H]-thymidine (final concentration: 0.025 mCi Elmer/New England Nuclear Corporation,,) was added for the last 18 hours of.<>Methyl-3[H]-thymidine uptake was measured as described
Molecular Function
Cytokine
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Preparation and Storage
The lyophilized protein is stable for at least years from date of receipt at-20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Application Data
(TGF-? suppresses DC production but has no effect on DC activation.Bar graphs summarize the average production of IL-12, IL-6, and IL-10 from CD11cdnR (n?=?4) and wild-type (n?=?4) cultures.)
Application Data
(TGF-? suppresses DC production but has no effect on DC activation.Bar graphs summarize the average production of IL-12, IL-6, and IL-10 from CD11cdnR (n?=?4) and wild-type (n?=?4) cultures.)
Application Data
(KJ1.26+ CD4+T cell purification and Th1 polarization of DO11.10 cells.Th1 polarization, then, was induced in purified cells, (c) IFN-? levels were measured in the supernatant of cocultures of DO11.10 CD4+ T cells, and macrophages, IL-12, and OVA323-339 peptide were used.)
Application Data
(KJ1.26+ CD4+T cell purification and Th1 polarization of DO11.10 cells.Th1 polarization, then, was induced in purified cells, (c) IFN-? levels were measured in the supernatant of cocultures of DO11.10 CD4+ T cells, and macrophages, IL-12, and OVA323-339 peptide were used.)
Application Data
(NR4A2 knockdown prevents IL-17 secretion but not ROR?t upregulation.A: IFN-? production by cells activated in the presence or absence of 10 ng/ml IL-12 after 96 hours of culture.)
Application Data
(NR4A2 knockdown prevents IL-17 secretion but not ROR?t upregulation.A: IFN-? production by cells activated in the presence or absence of 10 ng/ml IL-12 after 96 hours of culture.)
Application Data
(The IFN-? production from IL-18 and/or IL-12 stimulated Th1 cells from TRAM-deficient mice and MyD88 deficient mice.The black bars show the production levels from IL-18 and IL-12 co-stimulated Th1 cells, grey bars show those from IL-18 solely stimulated Th1 cells, and the white bars show those from no secondary stimulated Th1 cells.)
Application Data
(The IFN-? production from IL-18 and/or IL-12 stimulated Th1 cells from TRAM-deficient mice and MyD88 deficient mice.The black bars show the production levels from IL-18 and IL-12 co-stimulated Th1 cells, grey bars show those from IL-18 solely stimulated Th1 cells, and the white bars show those from no secondary stimulated Th1 cells.)
Application Data
(Increased splenic and hepatic DC numbers following systemic IL-12 treatment.BALB/c mice were treated with 1 µg/mouse IL-12 (solid square) or vehicle control (VC; open triangle) for 4 consecutive days from day 0, as indicated with arrows, then leukocyte populations analyzed at various days post treatment.)
Application Data
(Increased splenic and hepatic DC numbers following systemic IL-12 treatment.BALB/c mice were treated with 1 µg/mouse IL-12 (solid square) or vehicle control (VC; open triangle) for 4 consecutive days from day 0, as indicated with arrows, then leukocyte populations analyzed at various days post treatment.)
Application Data
(Exhausted CD8 T cells do not upregulate CD25 expression following IL-12, IL-18, and/or IL-21 exposure.Representative flow cytometry plots show the expression of CD25 by gated CD8 T cells following acute (B6 Arm), protracted (B6 cl13), and chronic (CD4-/- cl13) LCMV infections in response to a 5.5 hr incubation with or without IL-12, IL-18, and IL-21 in the absence of BFA.)
Application Data
(Exhausted CD8 T cells do not upregulate CD25 expression following IL-12, IL-18, and/or IL-21 exposure.Representative flow cytometry plots show the expression of CD25 by gated CD8 T cells following acute (B6 Arm), protracted (B6 cl13), and chronic (CD4-/- cl13) LCMV infections in response to a 5.5 hr incubation with or without IL-12, IL-18, and IL-21 in the absence of BFA.)
Product Categories/Family for Il12b active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
38,235 Da
NCBI Official Full Name
Interleukin-12 subunit beta
NCBI Official Synonym Full Names
interleukin 12b
NCBI Official Symbol
Il12b
NCBI Official Synonym Symbols
p40; Il-12b; Il12p40; Il-12p40
NCBI Protein Information
interleukin-12 subunit beta
UniProt Protein Name
Interleukin-12 subunit beta
UniProt Gene Name
Il12b
UniProt Synonym Gene Names
IL-12B; CLMF p40
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Product Notes
The Il12b il12b (Catalog #AAA14439) is an Active Protein produced from CHO cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.) and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MCPQKLTISW FAIVLLVSPL MAMWELEKDV YVVEVDWTPD APGETVNLTC DTPEEDDITW TSDQRHGVIG SGKTLTITVK EFLDAGQYTC HKGGETLSHS HLLLHKKENG IWSTEILKNF KNKTFLKCEA PNYSGRFTCS WLVQRNMDLK FNIKSSSSSP DSRAVTCGMA SLSAEKVTLD QRDYEKYSVS CQEDVTCPTA EETLPIELAL EARQQNKYEN YSTSFFIRDI IKPDPPKNLQ MKPLKNSQVE VSWEYPDSWS TPHSYFSLKF FVRIQRKKEK MKETEEGCNQ KGAFLVEKTS TEVQCKGGNV CVQAQDRYYN SSCSKWACVP CRVRS. It is sometimes possible for the material contained within the vial of "Interleukin-12 p40, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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