Mouse Chordin ELISA Kit | CHRD elisa kit

Mouse Chordin ELISA Kit

Cat. Num. AAA263111

• Gene Names: Chrd; Chd
• Reactivity: Mouse
• Synonyms: Chordin; N/A; Mouse Chordin ELISA Kit; CHRD elisa kit

• Reactivity: Mouse
• Specificity: The Mouse Chordin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse Chordin proteins within the range of 62.5 pg/ml - 4000 pg/ml.
• Sequence Length: 970
• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml

• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Standard Curve (Sample)

Related Product Information for CHRD elisa kit

Principle of the Assay: The Mouse Chordin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Chordin in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse Chordin coated on a 96-well plate. Standards and samples are pipetted into the wells and Chordin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Chordin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Chordin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Chordin is a key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins (BMPs) and sequestering them in latent complexes. In mice, Chordin is expressed in the node but not in the anterior visceral endoderm. It has been found to be required for forebrain development. In developing mice that are deficient in both chordin and noggin, the head is nearly absent. This is significant because when only noggin is deficient there are mild defects but the head still forms. Chordin is also involved in avian gastrulation. It is expressed in the anterior cells of Koller's sickle, which form the anterior cells of the primitive streak, a key structure through which gastrulation occurs.

NCBI and Uniprot Product Information

• NCBI GI #: 487442580
• NCBI GeneID: 12667
• NCBI Accession #: NP_001264970.1
• NCBI GenBank Nucleotide #: NM_001278041.1
• UniProt Accession #: Q9Z0E2
• Molecular Weight: 101,513 Da
• NCBI Official Full Name: chordin isoform 2
• NCBI Official Synonym Full Names: chordin
• NCBI Official Symbol: Chrd
• NCBI Official Synonym Symbols: Chd
• NCBI Protein Information: chordin
• UniProt Protein Name: Chordin
• UniProt Gene Name: Chrd
• UniProt Entry Name: CHRD_MOUSE