SDS-PAGE
Cholinergic Receptor, Nicotinic, Alpha 4 Recombinant Protein | CHRNa4 recombinant protein
Recombinant Cholinergic Receptor, Nicotinic, Alpha 4 (CHRNa4)
Gene Names
CHRNA4; EBN; BFNC; EBN1; NACHR; NACRA4; NACHRA4
Applications
Immunoprecipitation, ELISA, Western Blot, SDS-Page
Purity
> 95%
Synonyms
Cholinergic Receptor, Nicotinic, Alpha 4; N/A; Recombinant Cholinergic Receptor, Nicotinic, Alpha 4 (CHRNa4); CHR-NA4; CHRN-A4; BFNC; EBN; EBN1; NACRA4; N-AChRA4; NAChRA4; N-AChR-A4; Neuronal Acetylcholine Receptor Alpha 4; CHRNa4 recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Sequence
NKWSRPVANISD VVLVRFGLSI AQLIDVDEKN QMMTTNVWVK QEWHDYKLRWDPADYENVTS IRIPSELIWR PDIVLYNNAD GDFAVTHLTK AHLFHDGRVQWTPPAIYKSS CSIDVTFFPF DQQNCTMKFG SWTYDKAKID LVNMHSRVDQLDFWESGEWV IVDAVGTYNT RKYECCAEIY PDITYAFVIR RL
Sequence Length
627
Applicable Applications for CHRNa4 recombinant protein
IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE
Predicted Molecular Mass
26.5kDa
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Endotoxin Level
<1.0EU per 1 ug (determined by the LAL method)
Expression System
Prokaryotic expression
Fragment
Asn49~Leu242
Organism Species
Human
Tag
two N-terminal Tags, His-tag and T7-tag
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-PAGE
SDS-PAGE
Sequence
Sequence
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
60,994 Da
NCBI Official Full Name
Cholinergic receptor, nicotinic, alpha 4
NCBI Official Synonym Full Names
cholinergic receptor nicotinic alpha 4 subunit
NCBI Official Symbol
CHRNA4
NCBI Official Synonym Symbols
EBN; BFNC; EBN1; NACHR; NACRA4; NACHRA4
NCBI Protein Information
neuronal acetylcholine receptor subunit alpha-4
UniProt Protein Name
Neuronal acetylcholine receptor subunit alpha-4
UniProt Gene Name
CHRNA4
UniProt Synonym Gene Names
NACRA4
UniProt Entry Name
ACHA4_HUMAN
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Product Notes
The CHRNa4 chrna4 (Catalog #AAA145385) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cholinergic Receptor, Nicotinic, Alpha 4 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE. Researchers should empirically determine the suitability of the CHRNa4 chrna4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NKWSRPVANI SD VVLVRFGLSI AQLIDVDEKN QMMTTNVWVK QEWHDYKLRW DPADYENVTS IRIPSELIWR PDIVLYNNAD GDFAVTHLTK AHLFHDGRVQ WTPPAIYKSS CSIDVTFFPF DQQNCTMKFG SWTYDKAKID LVNMHSRVDQ LDFWESGEWV IVDAVGTYNT RKYECCAEIY PDITYAFVIR RL. It is sometimes possible for the material contained within the vial of "Cholinergic Receptor, Nicotinic, Alpha 4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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