Human Cold-inducible RNA-binding protein ELISA Kit | CIRBP elisa kit

Human Cold-inducible RNA-binding protein ELISA Kit

Cat. Num. AAA17447

• Gene Names: CIRBP; CIRP
• Reactivity: Human
• Synonyms: Cold-inducible RNA-binding protein; N/A; Human Cold-inducible RNA-binding protein ELISA Kit; CIRBP/Cold-inducible RNA-binding protein/A18 hnRNP/Glycine-rich RNA-binding protein CIRP/A18HNRNP/CIRP; CIRBP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CIRBP . No significant cross-reactivity or interference between CIRBP and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between CIRBP and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 297
• Assay Type: Sandwich ELISA, Double Antibody
• Samples: cell or tissue lysate, Other liquid samples
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CIRBP were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CIRBP were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for CIRBP elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CIRBP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CIRBP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CIRBP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CIRBP can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 664806037
• NCBI GeneID: 1153
• NCBI Accession #: NP_001287758.1
• NCBI GenBank Nucleotide #: NM_001300829.1
• Molecular Weight: 26,130 Da
• NCBI Official Full Name: cold-inducible RNA-binding protein isoform 3
• NCBI Official Synonym Full Names: cold inducible RNA binding protein
• NCBI Official Symbol: CIRBP
• NCBI Official Synonym Symbols: CIRP
• NCBI Protein Information: cold-inducible RNA-binding protein
• UniProt Protein Name: Cold-inducible RNA-binding protein
• UniProt Gene Name: CIRBP
• UniProt Synonym Gene Names: A18HNRNP; CIRP
• UniProt Entry Name: CIRBP_HUMAN