Standard Curve (Sample)
Human Cytokeratin 18 ELISA Kit | CK-18/KRT18 elisa kit
Human Cytokeratin 18 ELISA Kit
Gene Names
KRT18; K18; CK-18; CYK18
Reactivity
Human
Synonyms
Cytokeratin 18; N/A; Human Cytokeratin 18 ELISA Kit; KRT18/CK-18/Cytokeratin 18/Keratin; type I cytoskeletal 18/Keratin-18/K18/Cell proliferation-inducing gene 46 protein/Cytokeratin-18; CK-18/KRT18 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of CK-18/KRT18 . No significant cross-reactivity or interference between CK-18/KRT18 and analogues was observed.
Sequence Length
430
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
78.125-5000pg/ml
Sensitivity
46.875pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CK- 18/KRT18 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CK- 18/KRT18 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CK-18/KRT18 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CK- 18/KRT18 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CK- 18/KRT18 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CK-18/KRT18 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CK- 18/KRT18 can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
48,058 Da
NCBI Official Full Name
cytokeratin 18
NCBI Official Synonym Full Names
keratin 18, type I
NCBI Official Symbol
KRT18
NCBI Official Synonym Symbols
K18; CK-18; CYK18
NCBI Protein Information
keratin, type I cytoskeletal 18
UniProt Protein Name
Keratin, type I cytoskeletal 18
UniProt Gene Name
KRT18
UniProt Synonym Gene Names
CYK18; CK-18; K18
UniProt Entry Name
K1C18_HUMAN
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Product Notes
The Human CK-18/KRT18 krt18 (Catalog #AAA98782) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA98782 ELISA Kit recognizes Human CK-18/KRT18. It is sometimes possible for the material contained within the vial of "Cytokeratin 18, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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