Mouse Creatine Kinase ELISA Kit | CK elisa kit

Mouse Creatine Kinase ELISA Kit

Cat. Num. AAA87790

• Gene Names: CKMT1B; CKMT; CKMT1; UMTCK; CKMT1A
• Reactivity: Mouse
• Synonyms: Creatine Kinase; N/A; Mouse Creatine Kinase ELISA Kit; Mouse Creatine Kinase (CK) ELISA Kit; Creatine Kinase (Mouse); CK elisa kit

• Reactivity: Mouse
• Assay Type: Sandwich

• Preparation and Storage: Store all reagents at 2-8 degree C

Standard Curve (Sample)

Related Product Information for CK elisa kit

Intended Uses: This CK ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofCK in the sample, thisCK ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusCK concentration. The concentration ofCK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This CK enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forCK. Standards or samples are then added to the microtiter plate wells andCK if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofCK present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forCK are added to each well to "sandwich" theCK immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containCK and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

Product Categories/Family for CK elisa kit

Mouse ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 180590
• NCBI GeneID: 1159
• UniProt Accession #: P12532; Q0VAM3; Q32NF6; Q53FC4; B4DIT8; B7ZA09
• Molecular Weight: 47,037 Da
• NCBI Official Full Name: creatine kinase
• NCBI Official Synonym Full Names: creatine kinase, mitochondrial 1B
• NCBI Official Symbol: CKMT1B
• NCBI Official Synonym Symbols: CKMT; CKMT1; UMTCK; CKMT1A
• NCBI Protein Information: creatine kinase U-type, mitochondrial; U-MtCK; mia-CK; OTTHUMP00000066275; OTTHUMP00000200125; ubiquitous mitochondrial creatine kinase; acidic-type mitochondrial creatine kinase; creatine kinase, mitochondrial 1 (ubiquitous)
• UniProt Protein Name: Creatine kinase U-type, mitochondrial
• UniProt Gene Name: CKMT1A
• UniProt Synonym Gene Names: CKMT
• UniProt Entry Name: KCRU_HUMAN