SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
CK1 gamma 3 Recombinant Protein | CK1gamma3 recombinant protein
CK1 gamma 3, Active
Applications
Western Blot
Synonyms
CK1 gamma 3; N/A; CK1 gamma 3, Active; CSNK1G3; CK1gamma3 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for CK1gamma3 recombinant protein
WB (Western Blot), Kinase Assay
Tag
GST
Species
Human
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Related Product Information for CK1gamma3 recombinant protein
Full length recombinant human CK1gamma3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The CK1gamma3 gene accession number is NM_001031812.
CK1gamma3 is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes, including DNA replication and repair. CK1gamma3 is a ubiquitously expressed protein kinase found in the nuclei, cytoplasm and membrane fractions of eukaryotic cells (1). CK1gamma3 preferentially phosphorylates acidic substrates using ATP as a phosphate donor. The kinase domain of CKI isoforms have been shown to associate with protein kinase C-potentiated inhibitor protein of 17kDa, called CPI-17 (2). CPI-17 specifically inhibits myosin light chain phosphatase and this effect is potentiated when it is phosphorylated on Thr-38 by protein kinases such as the CK1 isoforms.
CK1gamma3 is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes, including DNA replication and repair. CK1gamma3 is a ubiquitously expressed protein kinase found in the nuclei, cytoplasm and membrane fractions of eukaryotic cells (1). CK1gamma3 preferentially phosphorylates acidic substrates using ATP as a phosphate donor. The kinase domain of CKI isoforms have been shown to associate with protein kinase C-potentiated inhibitor protein of 17kDa, called CPI-17 (2). CPI-17 specifically inhibits myosin light chain phosphatase and this effect is potentiated when it is phosphorylated on Thr-38 by protein kinases such as the CK1 isoforms.
Product Categories/Family for CK1gamma3 recombinant protein
References
1. Kusuda, J. et al: Cloning and chromosome mapping of the human casein kinase I gamma-3 gene (CSNK1G3). Cytogenet. Cell Genet. 83: 101-103, 1998.
2. Zemlickova, E. et al: Association of CPI-17 with protein kinase C and casein kinase I. Biochem Biophys Res Commun. 2004 Mar 26;316(1):39-47.
2. Zemlickova, E. et al: Association of CPI-17 with protein kinase C and casein kinase I. Biochem Biophys Res Commun. 2004 Mar 26;316(1):39-47.
NCBI and Uniprot Product Information
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Product Notes
The CK1gamma3 (Catalog #AAA73379) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CK1 gamma 3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Kinase Assay. Researchers should empirically determine the suitability of the CK1gamma3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CK1 gamma 3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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