Porcine Creatine Kinase, Mitochondrial 1A ELISA Kit | CKMT1A elisa kit

Porcine Creatine Kinase, Mitochondrial 1A ELISA Kit

Cat. Num. AAA88814

• Gene Names: CKMT1A; CKMT1
• Reactivity: Porcine
• Synonyms: Creatine Kinase, Mitochondrial 1A; N/A; Porcine Creatine Kinase, Mitochondrial 1A ELISA Kit; CKMT1A elisa kit

• Reactivity: Porcine
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Detection Range: 10-250 U/L
• Sensitivity: 1.0 U/L

• Intended Uses: This CKMT1A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine CKMT1A. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for CKMT1A elisa kit

Principle of the Assay: CKMTlAELISAkit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CKMT1 A. Standards or samples are then added to the microtiter plate wells and CKMT1A if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CKMT1A present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for CKMT1A are added to each well to "sandwich" the CKMT1A immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CKMT1A and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CKMT1A concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CKMT1A elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 66346730
• NCBI GeneID: 548596
• NCBI Accession #: NP_001015001.1
• NCBI GenBank Nucleotide #: NM_001015001.1
• Molecular Weight: 50,421 Da
• NCBI Official Full Name: creatine kinase U-type, mitochondrial
• NCBI Official Synonym Full Names: creatine kinase, mitochondrial 1A
• NCBI Official Symbol: CKMT1A
• NCBI Official Synonym Symbols: CKMT1
• NCBI Protein Information: creatine kinase U-type, mitochondrial; U-MtCK; mia-CK; ubiquitous mitochondrial creatine kinase; acidic-type mitochondrial creatine kinase; creatine kinase, mitochondrial 1 (ubiquitous)
• UniProt Protein Name: Creatine kinase U-type, mitochondrial
• UniProt Gene Name: CKMT1A
• UniProt Synonym Gene Names: CKMT; CKMT1B; CKMT; Mia-CK; U-MtCK
• UniProt Entry Name: KCRU_HUMAN