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product-image-AAA100153_SC13.jpg Standard Curve (Sample)

Mouse Collagen Type II ELISA Kit | COL2 elisa kit

Mouse Collagen Type II ELISA Kit

Gene Names
COL2A1; AOM; ANFH; SEDC; STL1; COL11A3
Reactivity
Mouse
Synonyms
Collagen Type II; N/A; Mouse Collagen Type II ELISA Kit; COL2 (Collagen Type II)/Chondrocalcin/COL2A1/SEDC/Alpha-1 type II collagen/ANFH/AOM/cartilage collagen/COL11A3/Collagen 2/collagen alpha-1 (II) chain/collagen II; alpha-1 polypeptide/collagen type 2; COL2 elisa kit
Ordering
Reactivity
Mouse
Sequence Length
1487
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
62.5-4000pg/ml
Sensitivity
37.5pg/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

product-image-AAA100153_SC13.jpg Standard Curve (Sample)

Standard Curve (Sample)

product-image-AAA100153_SC15.jpg Standard Curve (Sample)
Related Product Information for COL2 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse COL2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse COL2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse COL2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse COL2 can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
29,781 Da
NCBI Official Full Name
collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital), isoform CRA_e
NCBI Official Synonym Full Names
collagen type II alpha 1
NCBI Official Symbol
COL2A1
NCBI Official Synonym Symbols
AOM; ANFH; SEDC; STL1; COL11A3
NCBI Protein Information
collagen alpha-1(II) chain
UniProt Protein Name
Collagen alpha-1(II) chain
UniProt Gene Name
COL2A1
UniProt Entry Name
CO2A1_HUMAN

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Product Notes

The Mouse COL2 col2a1 (Catalog #AAA100153) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA100153 ELISA Kit recognizes Mouse COL2. It is sometimes possible for the material contained within the vial of "Collagen Type II, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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