Rat Collagen Type III ELISA Kit | COL3 elisa kit

Rat Collagen Type III ELISA Kit

Cat. Num. AAA17473

• Gene Names: COL3A1; EDS4A
• Reactivity: Rat
• Synonyms: Collagen Type III; N/A; Rat Collagen Type III ELISA Kit; COL3/COL3A1/EDS4A/COL3A1/EDS4A/alpha1 (III) collagen/Collagen 3/collagen alpha-1 (III) chain/collagen; fetal/collagen; type III; alpha 1/Collagen-3/EDS4A/Ehlers-Danlos syndrome type IV; autosomal dominant; COL3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of COL3. No significant cross-reactivity or interference between COL3 and analogues was observed.
• Sequence Length: 1466
• Assay Type: Sandwich ELISA, Double Antibody
• Samples: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
• Detection Range: 62.5-4000pg/ml
• Sensitivity: 37.5pg/ml

• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for COL3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 119631316
• NCBI GeneID: 1281
• Molecular Weight: 111,907 Da
• NCBI Official Full Name: collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant), isoform CRA_b
• NCBI Official Synonym Full Names: collagen type III alpha 1
• NCBI Official Symbol: COL3A1
• NCBI Official Synonym Symbols: EDS4A
• NCBI Protein Information: collagen alpha-1(III) chain
• UniProt Protein Name: Collagen alpha-1(III) chain
• UniProt Gene Name: COL3A1
• UniProt Entry Name: CO3A1_HUMAN