Standard Curve (Sample)
Mouse Collagen Type III Alpha 1 ELISA Kit | COL3a1 elisa kit
Mouse Collagen Type III Alpha 1 ELISA Kit
Gene Names
COL3A1; EDS4A
Reactivity
Mouse
Synonyms
Collagen Type III Alpha 1; Mouse Collagen Type III Alpha 1 ELISA Kit; COL3a1 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of COL3alpha1. No significant cross-reactivity or interference between COL3alpha1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between COL3alpha1 and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for COL3a1 elisa kit
Principle of the Assay: COL3alpha1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-COL3alpha1 antibody and an COL3alpha1-HRP conjugate. The assay sample and buffer are incubated together with COL3alpha1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COL3alpha1 concentration since COL3alpha1 from samples and COL3alpha1-HRP conjugate compete for the anti-COL3alpha1 antibody binding site. Since the number of sites is limited, as more sites are occupied by COL3alpha1 from the sample, fewer sites are left to bind COL3alpha1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL3alpha1 concentration in each sample is interpolated from this standard curve.
Intended Uses: This COL3alpha1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse COL3alpha1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This COL3alpha1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse COL3alpha1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for COL3a1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
111,907 Da
NCBI Official Full Name
collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant), isoform CRA_b
NCBI Official Synonym Full Names
collagen, type III, alpha 1
NCBI Official Symbol
COL3A1
NCBI Official Synonym Symbols
EDS4A
NCBI Protein Information
collagen alpha-1(III) chain; collagen alpha-1(III) chain; collagen, fetal; alpha1 (III) collagen; Ehlers-Danlos syndrome type IV, autosomal dominant
UniProt Protein Name
Collagen alpha-1(III) chain
UniProt Gene Name
COL3A1
UniProt Entry Name
CO3A1_HUMAN
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Product Notes
The Mouse COL3a1 col3a1 (Catalog #AAA16249) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA16249 ELISA Kit recognizes Mouse COL3a1. It is sometimes possible for the material contained within the vial of "Collagen Type III Alpha 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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