Standard Curve (Sample)
Human collagen, type IV, alpha 3 (Goodpasture antigen) ELISA Kit | COL4A3 elisa kit
Human Collagen alpha-3 (IV) chain, COL4A3 ELISA Kit
Reactivity
Human
Synonyms
collagen, type IV, alpha 3 (Goodpasture antigen); N/A; Human Collagen alpha-3 (IV) chain, COL4A3 ELISA Kit; Human Collagen alpha-3 (IV) chain (COL4A3) ELISA kit; alpha 3 type IV collagen; collagen IV; alpha-3 polypeptide; tumstatin; collagen; type IV; alpha 3 (Goodpasture antigen); COL4A3 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human COL4A3. No significant cross-reactivity or interference between human COL4A3 and analogues was observed.
Sequence Length
1670
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.156 ng/ml-10 ng/ml
Sensitivity
< 0.039 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for COL4A3 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for COL4A3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COL4A3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COL4A3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COL4A3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
161,813 Da
NCBI Official Full Name
collagen alpha-3(IV) chain
NCBI Official Synonym Full Names
collagen, type IV, alpha 3 (Goodpasture antigen)
NCBI Official Symbol
COL4A3
NCBI Protein Information
collagen alpha-3(IV) chain; collagen alpha-3(IV) chain; tumstatin; collagen IV, alpha-3 polypeptide
UniProt Protein Name
Collagen alpha-3(IV) chain
UniProt Gene Name
COL4A3
UniProt Entry Name
CO4A3_HUMAN
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Product Notes
The Human COL4A3 col4a3 (Catalog #AAA18165) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA18165 ELISA Kit recognizes Human COL4A3. It is sometimes possible for the material contained within the vial of "collagen, type IV, alpha 3 (Goodpasture antigen), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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