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product-image-AAA76123_AD11.jpg Application Data

COVID 19 Spike S Coronavirus Recombinant Protein | COVID-19 recombinant protein

SARS COV2 Spike protein S Recombinant-Fc (AVI tag)

Purity
>95% by SDS-PAGE
Synonyms
COVID 19 Spike S Coronavirus; N/A; SARS COV2 Spike protein S Recombinant-Fc (AVI tag); 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike S; AVI tag; COVID-19 recombinant protein
Ordering
Host
HEK Cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Purity/Purification
>95% by SDS-PAGE
Form/Format
Recombinant nCoV-2019 Spike S protein was lyophilized from 0.2 µm filtered PBS pH 7.4
Sequence Positions
14-1211
Source Note
DNA sequence encoding the COVID-19 Spike S protein extracellular domain,[14-1211] accession #QHD43416.1 including a C-terminal Fc AVI tag was expressed in HEK Cells. (The furin protease cleavage site was mutated at position 683)
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug)
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C

Application Data

product-image-AAA76123_AD11.jpg Application Data

HPLC

(The purity of 2019-nCoV Spike S is greater than 95% as determined by SEC-HPLC.)

product-image-AAA76123_HPLC13.jpg HPLC (The purity of 2019-nCoV Spike S is greater than 95% as determined by SEC-HPLC.)

PAGE

(1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.)

product-image-AAA76123_PAGE15.jpg PAGE (1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.)

NCBI and Uniprot Product Information

Molecular Weight
Recombinant 2019-nCoV Spike S protein is a protein consisting of the S1+S2 subunits with a calculated MW of 160kDa ,due to glycosylation migrates as an approximately 220 kDa protein on SDS-PAGE.

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Product Notes

The COVID-19 (Catalog #AAA76123) is a Recombinant Protein produced from HEK Cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders) and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 14-1211. It is sometimes possible for the material contained within the vial of "COVID 19 Spike S Coronavirus, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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