CD21 Antibody | anti-CD21 antibody

Anti-Hu CD21 APC-Cy7

Cat. Num. AAA129280

• Gene Names: CR2; CR; C3DR; CD21; CVID7; SLEB9
• Reactivity: Pig, Human, Dog, Cow
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD21; N/A; Anti-Hu CD21 APC-Cy7; complement C3d receptor 2; CR2; C3DR2; CVID7; SLEB9; anti-CD21 antibody

• Reactivity: Pig, Human, Dog, Cow
• Isotype: Mouse IgG1
• Clone Number: LT21
• Specificity: The antibody LT21 reacts with an extracellular epitope of CD21 (CR2), a 145 kDa transmembrane glycoprotein (complement C3d receptor - C3dR) expressed on B lymphocytes, follicular dendritic cells, some epithelial cells and a subsets of T lymphocytes. It is not expressed on immature B cells.
• Form/Format: APC-Cy7; Purified antibody is conjugated with activated tandem dye of allophycocyanin-cyanine 7 (APC-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.; Stabilizing phosphate buf

• Applicable Applications for anti-CD21 antibody: FCM/FACS (Flow Cytometry)
• Immunogen: IM9 human B-lymphoblastoid cell line
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human CD21 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD21 (LT21) APC-Cy™7 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD21 (LT21) APC-Cy™7 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)

Related Product Information for anti-CD21 antibody

CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.

References

*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., URL:,*Faldyna M, Samankova P, Leva L, Cerny J, Oujezdska J, Rehakova Z, Sinkora J: Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs. Vet Immunol Immunopathol. 2007 Sep 15;119(1-2):56-62., URL: https://www.ncbi.nlm.nih.gov/pubmed/17673300?ordin,*Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry. J Immunol Methods. 2007 Jan 30;319(1-2):21-33., URL: http://www.ncbi.nlm.nih.gov/pubmed/17098248,
*Singh A, Blank M, Shoenfeld Y, Illges H: Antiphospholipid syndrome patients display reduced titers of soluble CD21 in their sera irrespective of circulating anti-beta2-glycoprotein-I autoantibodies. Rheumatol Int. 2008 Jan 3, URL: https://www.ncbi.nlm.nih.gov/pubmed/18172655?ordin,*Zabel MD, Heikenwalder M, Prinz M, Arrighi I, Schwarz P, Kranich J, von Teichman A, Haas KM, Zeller N, Tedder TF, Weis JH, Aguzzi A: Stromal complement receptor CD21/35 facilitates lymphoid prion colonization and pathogenesis. J Immunol. 2007 Nov 1;179(9):6144-52., URL: https://www.ncbi.nlm.nih.gov/pubmed/17947689?ordin,*Kasprzak A, Spachacz R, Wachowiak J, Stefanska K, Zabel M: Epstein-Barr virus (EBV) infection in B-cell non-Hodgkin's lymphomas in children: virus latency and its correlation with CD21 and CD23 molecules. Folia Histochem Cytobiol. 2007;45(3):169-79., URL: https://www.ncbi.nlm.nih.gov/pubmed/17951165?ordin,*Twohig J, Kulik L, Haluszczak C, Reuter J, Rossbach A, Bull M, Holers VM, Marchbank KJ: Defective B cell ontogeny and immune response in human complement receptor 2 (CR2, CD21) transgenic mice is partially recovered in the absence of C3. Mol Immunol. 2007 Jul;44(13):3434-44., URL: https://www.ncbi.nlm.nih.gov/pubmed/17379312?ordin,*Roozendaal R, Carroll MC: Complement receptors CD21 and CD35 in humoral immunity. Immunol Rev. 2007 Oct;219:157-66., URL: https://www.ncbi.nlm.nih.gov/pubmed/17850488?ordin,

NCBI and Uniprot Product Information

• NCBI GI #: 215273962
• NCBI GeneID: 1380
• UniProt Accession #: P20023
• NCBI Official Full Name: Complement receptor type 2
• NCBI Official Synonym Full Names: complement C3d receptor 2
• NCBI Official Symbol: CR2
• NCBI Official Synonym Symbols: CR; C3DR; CD21; CVID7; SLEB9
• NCBI Protein Information: complement receptor type 2
• UniProt Protein Name: Complement receptor type 2
• UniProt Gene Name: CR2
• UniProt Synonym Gene Names: C3DR; Cr2; EBV receptor
• UniProt Entry Name: CR2_HUMAN