Bovine Creatine phosphokinase ELISA Kit | CPK elisa kit
Bovine Creatine phosphokinase ELISA Kit
Reactivity
Bovine
Synonyms
Creatine phosphokinase; N/A; Bovine Creatine phosphokinase ELISA Kit; CPK elisa kit
Reactivity
Bovine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
5.0-100ng/mL
Sensitivity
0.1ng/mL
Intended Uses
This CP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine CP. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for CPK elisa kit
Principle of the assay: CP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CP antibody and an CP-HRP conjugate. The assay sample and buffer are incubated together with CP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CP concentration since CP from samples and CP-HRP conjugate compete for the anti-CP antibody binding site. Since the number of sites is limited, as more sites are occupied by CP from the sample, fewer sites are left to bind CP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,112 Da
NCBI Official Full Name
creatine kinase M-type
NCBI Official Symbol
CKM
NCBI Protein Information
creatine kinase M-type; M-CK; muscle creatine kinase; creatine kinase M chain
UniProt Protein Name
Creatine kinase M-type
UniProt Gene Name
CKM
UniProt Entry Name
KCRM_RABIT
