Mouse Carnitine palmitoyltransferase I ELISA Kit | CPT-1 elisa kit

Mouse Carnitine palmitoyltransferase I ELISA Kit

Cat. Num. AAA16152

• Gene Names: Cpt1a; CPT-Ia
• Reactivity: Mouse
• Synonyms: Carnitine palmitoyltransferase I; N/A; Mouse Carnitine palmitoyltransferase I ELISA Kit; CPT-1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of CPT1. No significant cross-reactivity or interference between CPT1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CPT1 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for CPT-1 elisa kit

Principle of the Assay: CPT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CPT1 antibody and an CPT1-HRP conjugate. The assay sample and buffer are incubated together with CPT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPT1 concentration since CPT1 from samples and CPT1-HRP conjugate compete for the anti-CPT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CPT1 from the sample, fewer sites are left to bind CPT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPT1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This CPT1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CPT1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

• NCBI GI #: 146345405
• NCBI GeneID: 25757
• Molecular Weight: 88,125 Da
• NCBI Official Full Name: Carnitine O-palmitoyltransferase 1, liver isoform
• NCBI Official Synonym Full Names: carnitine palmitoyltransferase 1a, liver
• NCBI Official Symbol: Cpt1a
• NCBI Official Synonym Symbols: CPT-Ia
• NCBI Protein Information: carnitine O-palmitoyltransferase 1, liver isoform; CPT I; CPT1-L; CPTI-L; carnitine palmitoyltransferase 1, liver; carnitine O-palmitoyltransferase I, liver isoform; Carnitine palmitoyltransferase 1 alpha, liver isoform
• UniProt Protein Name: Carnitine O-palmitoyltransferase 1, liver isoform
• UniProt Gene Name: Cpt1a
• UniProt Synonym Gene Names: Cpt-1; Cpt1; CPT1-L; CPT I; CPTI-L
• UniProt Entry Name: CPT1A_RAT