Human Collapsin response mediator protein 5 ELISA Kit | CRMP5 elisa kit
Human Collapsin response mediator protein 5 ELISA Kit
Gene Names
DPYSL5; CRAM; CRMP5; Ulip6; CRMP-5
Reactivity
Human
Synonyms
Collapsin response mediator protein 5; N/A; Human Collapsin response mediator protein 5 ELISA Kit; CRMP5 elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
5.0-100ng/mL
Sensitivity
1.0ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for CRMP5 elisa kit
Intended Uses||This CRMP5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CRMP5. This ELISA kit for research use only!
Principle of the Assay: CRMP5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CRMP5 antibody and an CRMP5-HRP conjugate. The assay sample and buffer are incubated together with CRMP5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme- substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CRMP5 concentration since CRMP5 from samples and CRMP5- HRP conjugate compete for the anti- CRMP5 antibody binding site. Since the number of sites is limited, as more sites are occupied by CRMP5 from the sample, fewer sites are left to bind CRMP5-HRP conjugate. A standards curve is plotted relating the intensity of the color (O.D.) to the concentration standard curve.
Principle of the Assay: CRMP5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CRMP5 antibody and an CRMP5-HRP conjugate. The assay sample and buffer are incubated together with CRMP5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme- substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CRMP5 concentration since CRMP5 from samples and CRMP5- HRP conjugate compete for the anti- CRMP5 antibody binding site. Since the number of sites is limited, as more sites are occupied by CRMP5 from the sample, fewer sites are left to bind CRMP5-HRP conjugate. A standards curve is plotted relating the intensity of the color (O.D.) to the concentration standard curve.
Product Categories/Family for CRMP5 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
61,421 Da
NCBI Official Full Name
collapsin response mediator protein-5
NCBI Official Synonym Full Names
dihydropyrimidinase-like 5
NCBI Official Symbol
DPYSL5
NCBI Official Synonym Symbols
CRAM; CRMP5; Ulip6; CRMP-5
NCBI Protein Information
dihydropyrimidinase-related protein 5; DRP-5; ULIP-6; CRMP3-associated molecule; UNC33-like phosphoprotein 6; collapsin response mediator protein 5; collapsin response mediator protein-5
UniProt Protein Name
Dihydropyrimidinase-related protein 5
UniProt Gene Name
DPYSL5
UniProt Synonym Gene Names
CRMP5; ULIP6; DRP-5; CRAM; CRMP-5; ULIP-6
UniProt Entry Name
DPYL5_HUMAN
