SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
CRYGD recombinant protein
CRYGD, 1-174aa, Human, His tag, E Coli
Gene Names
CRYGD; CCP; PCC; CACA; CCA3; CRYG4; CTRCT4; cry-g-D
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
CRYGD; N/A; CRYGD, 1-174aa, Human, His tag, E Coli; Gamma-crystallin D; CACA; CCA3; CCP; cry-g-D; CRYG4; CRYGD recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 5mM DTT, 10% glycerol, 200mM NaCl
Concentration
1mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
1-174aa
Sequence
MGSSHHHHHH SSGLVPRGSH MGKITLYEDR GFQGRHYECS SDHPNLQPYL SRCNSARVDS GCWMLYEQPN YSGLQYFLRR GDYADHQQWM GLSDSVRSCR LIPHSGSHRI RLYEREDYRG QMIEFTEDCS CLQDRFRFNE IHSLNVLEGS WVLYELSNYR GRQYLLMPGD YRRYQDWGAT NARVGSLRRV IDFS
Applicable Applications for CRYGD recombinant protein
SDS-PAGE
Tag Information
His-tag
Preparation and Storage
Can be stored at 2-8°C for 1 week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing.
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for CRYGD recombinant protein
CRYGD belongs to the beta/gamma-crystallin family. Crystallins are the dominant structural components of the vertebrate eye lens. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Gamma-crystallins have been involved in cataract formation. Defects in CRYGD are a cause of cataract autosomal dominant (ADC), cataract congenital non-nuclear polymorphic autosomal dominant (CCP), cataract congenital cerulean type 3 (CCA3) and cataract crystalline aculeiform (CACA). Recombinant human CRYGD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Categories/Family for CRYGD recombinant protein
References
Pande A., et al. (2001) Proc. Natl. Acad. Sci. u.S.A. 98:6116-6120; Plotnikova O.V., et al. (2007) Am. J. Hum. Genet. 81:32-43
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22.9kDa (194a), confirmed by MALDI-TOF
NCBI Official Full Name
gamma-crystallin D
NCBI Official Synonym Full Names
crystallin gamma D
NCBI Official Symbol
CRYGD
NCBI Official Synonym Symbols
CCP; PCC; CACA; CCA3; CRYG4; CTRCT4; cry-g-D
NCBI Protein Information
gamma-crystallin D
UniProt Protein Name
Gamma-crystallin D
UniProt Gene Name
CRYGD
UniProt Synonym Gene Names
CRYG4
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Product Notes
The CRYGD crygd (Catalog #AAA11809) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-174aa with tag His-tag. AAA Biotech's CRYGD can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the CRYGD crygd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGKITLYEDR GFQGRHYECS SDHPNLQPYL SRCNSARVDS GCWMLYEQPN YSGLQYFLRR GDYADHQQWM GLSDSVRSCR LIPHSGSHRI RLYEREDYRG QMIEFTEDCS CLQDRFRFNE IHSLNVLEGS WVLYELSNYR GRQYLLMPGD YRRYQDWGAT NARVGSLRRV IDFS. It is sometimes possible for the material contained within the vial of "CRYGD, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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