Rat Cystatin C ELISA Kit | CST3 elisa kit

Rat Cystatin C PicoKine ELISA Kit

Cat. Num. AAA45856

• Gene Names: Cst3; CYSC
• Reactivity: Rat
• Synonyms: Cystatin C; N/A; Rat Cystatin C PicoKine ELISA Kit; Cystatin-C; cystatin C; Cst3; Cystatin-3; CYTC_HUMAN; Gamma-trace; Neuroendocrine basic polypeptide; Post-gamma-globulin; CST3 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat Cystatin C
• Sequence Length: 140
• Samples: Cell culture supernates, serum, plasma (heparin, EDTA) and urine
• Assay Type: Sandwich
• Detection Range: 312pg/ml-20,000pg/ml
• Sensitivity: < 10pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Rat Cystatin C PicoKine ELISA Kit standard curve)

Related Product Information for CST3 elisa kit

Principle of the assay: rat Cystatin C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cystatin C has been precoated onto 96-well plates. Standards(NSO, M1-A140) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cystatin C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Cystatin C amount of sample captured in plate.
Background: Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure. Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In mouses, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.

NCBI and Uniprot Product Information

• NCBI GI #: 83301921
• NCBI GeneID: 25307
• UniProt Accession #: P14841; Q5M968
• Molecular Weight: 15,437 Da
• NCBI Official Full Name: Cystatin-C
• NCBI Official Synonym Full Names: cystatin C
• NCBI Official Symbol: Cst3
• NCBI Official Synonym Symbols: CYSC
• NCBI Protein Information: cystatin-C; cystatin 3; cystatin-3; Cystatin C (cysteine proteinase inhibitor)
• UniProt Protein Name: Cystatin-C
• UniProt Gene Name: Cst3
• UniProt Entry Name: CYTC_RAT