Mouse Cathepsin B ELISA Kit | CTSB elisa kit

Mouse Cathepsin B PicoKine ELISA Kit

Cat. Num. AAA11528

• Gene Names: Ctsb; CB
• Reactivity: Mouse
• Synonyms: Cathepsin B; N/A; Mouse Cathepsin B PicoKine ELISA Kit; cathepsin B; aAmyloid precursor protein secretase; APP secretase; APPS; CATB_HUMAN; Cathepsin B heavy chain; Cathepsin B1; CPSB; CTSB; Cysteine protease; CTSB elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Ctsb
• Sequence Length: 339
• Samples: Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 156 pg/ml - 10,000 pg/ml
• Sensitivity: <5 pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

Related Product Information for CTSB elisa kit

Background: Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene. 1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease. 4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant mouse Cathepsin B with the molecular mass of 37KDa.

Principle of the Assay: The Picokine™ Mouse Ctsb Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Ctsb with a 96-well strip plate that is pre-coated with antibody specific for Ctsb. The detection antibody is a biotinylated antibody specific for Ctsb. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit includes Mouse Ctsb protein as standards. To measure Mouse Ctsb, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Ctsb in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Ctsb in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 115712
• NCBI GeneID: 13030
• UniProt Accession #: P10605; Q3UDW7
• Molecular Weight: 37,280 Da
• NCBI Official Full Name: Cathepsin B
• NCBI Official Synonym Full Names: cathepsin B
• NCBI Official Symbol: Ctsb
• NCBI Official Synonym Symbols: CB
• NCBI Protein Information: cathepsin B; cathepsin B1
• UniProt Protein Name: Cathepsin B
• UniProt Gene Name: Ctsb
• UniProt Entry Name: CATB_MOUSE